Methane oxidising bacteria to upcycle effluent streams from anaerobic digestion of municipal biowaste

Panagiotis Tsapekos, Benyamin Khoshnevisan, Xinyu Zhu, Xiao Zha, Irini Angelidaki*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Conventional microbial protein production relies on the usage of pure chemicals and gases. Natural gas, which is a fossil resource, is the common input gas for bacterial protein production. Alternative sources for gas feedstock and nutrients can sufficiently decrease the operational cost and environmental impact of microbial protein production processes. In the present study, the effluents streams of municipal biowaste anaerobic digestion, were used to grow methane oxidising bacteria which can be used as protein source. Results demonstrated that a 40:60 CH4:O2 (v/v) gas feeding resulted in microbial biomass production of 0.95 g-DM/L by a Methylophilus dominated community. When raw biogas was used as input for methane corresponding to the same initial methane partial pressure as before, instead of pure methane, the growth was partially hindered (0.61 g-DM/L) due to the presence of H2S (IC50: 1376 ppm). Hence, desulfurization is suggested before using biogas for microbial protein production. At semi-continuous mode, results showed that the produced biomass had relatively high protein content (>40% of dry weight) and the essential amino acids lysine, valine, leucine and histidine were detected at high levels.
Original languageEnglish
Article number109590
JournalJournal of Environmental Management
Volume251
Number of pages7
ISSN0301-4797
DOIs
Publication statusPublished - 2019

Keywords

  • Methanotrophs
  • Mixed culture
  • Minicipal biowaste
  • Digestate
  • 16S rRNA

Cite this

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title = "Methane oxidising bacteria to upcycle effluent streams from anaerobic digestion of municipal biowaste",
abstract = "Conventional microbial protein production relies on the usage of pure chemicals and gases. Natural gas, which is a fossil resource, is the common input gas for bacterial protein production. Alternative sources for gas feedstock and nutrients can sufficiently decrease the operational cost and environmental impact of microbial protein production processes. In the present study, the effluents streams of municipal biowaste anaerobic digestion, were used to grow methane oxidising bacteria which can be used as protein source. Results demonstrated that a 40:60 CH4:O2 (v/v) gas feeding resulted in microbial biomass production of 0.95 g-DM/L by a Methylophilus dominated community. When raw biogas was used as input for methane corresponding to the same initial methane partial pressure as before, instead of pure methane, the growth was partially hindered (0.61 g-DM/L) due to the presence of H2S (IC50: 1376 ppm). Hence, desulfurization is suggested before using biogas for microbial protein production. At semi-continuous mode, results showed that the produced biomass had relatively high protein content (>40{\%} of dry weight) and the essential amino acids lysine, valine, leucine and histidine were detected at high levels.",
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Methane oxidising bacteria to upcycle effluent streams from anaerobic digestion of municipal biowaste. / Tsapekos, Panagiotis; Khoshnevisan, Benyamin; Zhu, Xinyu; Zha, Xiao; Angelidaki, Irini.

In: Journal of Environmental Management, Vol. 251, 109590, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Khoshnevisan, Benyamin

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AU - Zha, Xiao

AU - Angelidaki, Irini

PY - 2019

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AB - Conventional microbial protein production relies on the usage of pure chemicals and gases. Natural gas, which is a fossil resource, is the common input gas for bacterial protein production. Alternative sources for gas feedstock and nutrients can sufficiently decrease the operational cost and environmental impact of microbial protein production processes. In the present study, the effluents streams of municipal biowaste anaerobic digestion, were used to grow methane oxidising bacteria which can be used as protein source. Results demonstrated that a 40:60 CH4:O2 (v/v) gas feeding resulted in microbial biomass production of 0.95 g-DM/L by a Methylophilus dominated community. When raw biogas was used as input for methane corresponding to the same initial methane partial pressure as before, instead of pure methane, the growth was partially hindered (0.61 g-DM/L) due to the presence of H2S (IC50: 1376 ppm). Hence, desulfurization is suggested before using biogas for microbial protein production. At semi-continuous mode, results showed that the produced biomass had relatively high protein content (>40% of dry weight) and the essential amino acids lysine, valine, leucine and histidine were detected at high levels.

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