Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

Indumathi Vedarethinam, Pranjul Jaykumar Shah, Maria Dimaki, Z. Tumer, N. Tommerup, Winnie Edith Svendsen

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    Abstract

    Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3-5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies.
    Original languageEnglish
    JournalSensors
    Volume10
    Issue number11
    Pages (from-to)9831-9846
    ISSN1424-8220
    DOIs
    Publication statusPublished - 2010

    Bibliographical note

    This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

    Keywords

    • chromosome spreading
    • chromosomal translocations
    • genetic analysis
    • cytogenetics
    • lab on chip
    • Fluorescence in situ Hybridization (FISH)
    • metaphase FISH

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