Metagenomics and single-cell genomics reveal high abundance of comammox Nitrospira in a rapid gravity sand filter treating groundwater

Alejandro Palomo, Jane Fowler, Arda Gülay, Simon Rasmussen, Andreas Schramm, Thomas Sicheritz-Pontén, Barth F. Smets

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

278 Downloads (Pure)

Abstract

The recent discovery of complete ammonia oxidizing (comammox) Nitrospira has revealed that the metabolic division of labor in nitrification is not obligate as was assumed during the last century. Despite the detection and enrichment of commamox Nitrospira from different nitrifying environments, the ecological relevance of comammox remains unknown. In this study, we analyzed the microbial communities from various locations within a groundwater-fed rapid sand filter (RSF), where Nitrospira were at very high relative abundances. Through metagenomics, a highly abundant composite multi-genome of Nitrospira genus was recovered harboring metabolic capacity for complete ammonia oxidation. We developed a cell extraction strategy that enables the disruption of Nitrospira cell clusters attached to the mineral coating of the sand. Individual cells were identified via fluorescent in situ hybridization (FISH) with Nitrospira-specific 16S rRNA probes and sorted via fluorescence-activated cell sorting (FACS). Sorted cells were screened and selected Nitrospira spp. were subject to whole-genome sequencing. The single cell genomes confirmed the genomic presence of a complete ammonia oxidation pathway and revealed clear taxonomic differences with the recently described comammox Nitrospira genomes. The high abundance of comammox Nitrospira spp. together with the low abundance of canonical ammonia oxidizing prokaryotes in the investigated RSF system suggests the essential role of this novel comammox Nitrospira in the RSFs and potentially other nitrifying environments.
Original languageEnglish
Publication date2016
Number of pages1
Publication statusPublished - 2016
Event16th International Symposium on Microbial Ecology - Montreal, Canada
Duration: 21 Aug 201626 Aug 2016
Conference number: 16
http://www.isme-microbes.org/isme16

Conference

Conference16th International Symposium on Microbial Ecology
Number16
CountryCanada
CityMontreal
Period21/08/201626/08/2016
Internet address

Fingerprint Dive into the research topics of 'Metagenomics and single-cell genomics reveal high abundance of comammox Nitrospira in a rapid gravity sand filter treating groundwater'. Together they form a unique fingerprint.

Cite this