Metagenomic binning reveals the functional roles of core abundant microorganisms in twelve full-scale biogas plants

Stefano Campanaro, Laura Treu, Panagiotis Kougias*, Gang Luo, Irini Angelidaki

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The aim of this work was to elucidate the microbial ecology in twelve mesophilic and thermophilic full-scale biogas plants using a genome-centric metagenomic approach. In this study both biogas plants treating manure and those treating sludge from waste water treatment plants were considered. The identification of 132 Metagenome-Assembled Genomes (MAGs) and analysis of their abundance profile in different samples allowed the identification of the most abundant core members of the anaerobic digestion microbiome. Canonical correspondence analysis was used to determine the influence of biotic and environmental factors on MAGs abundance and to investigate the methanogenic performance of the biogas plants. Prediction of the functional properties of MAGs was obtained analyzing their KEGG pathways and their carbohydrate active domains. Network analysis allowed investigation of species-species associations and shed light on syntrophic interactions between members belonging to the anaerobic digestion dark matter (phylum Fermentibacteria). By stratifying and comparing different levels of information, it was predicted that some MAGs have a crucial role in the manure-supplemented thermophilic biogas plants and it was highlighted the importance of the glycine cleavage system in complementing the "truncated" Wood-Ljungdahl pathway.
Original languageEnglish
JournalWater Research
Volume140
Pages (from-to)123-134
ISSN0043-1354
DOIs
Publication statusPublished - 2018

Keywords

  • Abundant metagenome-assembled genomes
  • Anaerobic digestion
  • Functional analysis
  • Metagenomic binning

Cite this

Campanaro, Stefano ; Treu, Laura ; Kougias, Panagiotis ; Luo, Gang ; Angelidaki, Irini. / Metagenomic binning reveals the functional roles of core abundant microorganisms in twelve full-scale biogas plants. In: Water Research. 2018 ; Vol. 140. pp. 123-134.
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abstract = "The aim of this work was to elucidate the microbial ecology in twelve mesophilic and thermophilic full-scale biogas plants using a genome-centric metagenomic approach. In this study both biogas plants treating manure and those treating sludge from waste water treatment plants were considered. The identification of 132 Metagenome-Assembled Genomes (MAGs) and analysis of their abundance profile in different samples allowed the identification of the most abundant core members of the anaerobic digestion microbiome. Canonical correspondence analysis was used to determine the influence of biotic and environmental factors on MAGs abundance and to investigate the methanogenic performance of the biogas plants. Prediction of the functional properties of MAGs was obtained analyzing their KEGG pathways and their carbohydrate active domains. Network analysis allowed investigation of species-species associations and shed light on syntrophic interactions between members belonging to the anaerobic digestion dark matter (phylum Fermentibacteria). By stratifying and comparing different levels of information, it was predicted that some MAGs have a crucial role in the manure-supplemented thermophilic biogas plants and it was highlighted the importance of the glycine cleavage system in complementing the {"}truncated{"} Wood-Ljungdahl pathway.",
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author = "Stefano Campanaro and Laura Treu and Panagiotis Kougias and Gang Luo and Irini Angelidaki",
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Metagenomic binning reveals the functional roles of core abundant microorganisms in twelve full-scale biogas plants. / Campanaro, Stefano; Treu, Laura; Kougias, Panagiotis ; Luo, Gang; Angelidaki, Irini.

In: Water Research, Vol. 140, 2018, p. 123-134.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Angelidaki, Irini

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AB - The aim of this work was to elucidate the microbial ecology in twelve mesophilic and thermophilic full-scale biogas plants using a genome-centric metagenomic approach. In this study both biogas plants treating manure and those treating sludge from waste water treatment plants were considered. The identification of 132 Metagenome-Assembled Genomes (MAGs) and analysis of their abundance profile in different samples allowed the identification of the most abundant core members of the anaerobic digestion microbiome. Canonical correspondence analysis was used to determine the influence of biotic and environmental factors on MAGs abundance and to investigate the methanogenic performance of the biogas plants. Prediction of the functional properties of MAGs was obtained analyzing their KEGG pathways and their carbohydrate active domains. Network analysis allowed investigation of species-species associations and shed light on syntrophic interactions between members belonging to the anaerobic digestion dark matter (phylum Fermentibacteria). By stratifying and comparing different levels of information, it was predicted that some MAGs have a crucial role in the manure-supplemented thermophilic biogas plants and it was highlighted the importance of the glycine cleavage system in complementing the "truncated" Wood-Ljungdahl pathway.

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