Metagenomic analysis of bacterial community composition in Dhanaan: Ethiopian traditional fermented camel milk

Tesfemariam Berhe*, Richard Ipsen, Eyassu Seifu, Mohamed Y Kurtu, Angelina June Brandt Fugl, Egon Bech Hansen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

This study was conducted to evaluate the safety and bacterial profile of Dhanaan (Ethiopian traditional fermented camel milk). The composition of the microbial community in Dhanaan samples was analysed by a metagenomic approach of 16S rRNA gene amplicon sequencing. Metagenomic profiling identified 87 different bacterial microorganisms (OTUs) in six samples analysed. Although the Dhanaan samples contained various lactic acid bacteria (LAB), they also all contained undesirable microorganisms in large proportions. The following LAB genera were identified: Streptococcus, Lactococcus, and Weissella. One Streptococcus species represented by OTU-1 (operational taxonomic unit) was found in all Dhanaan samples and the dominating species in four out of six samples. This common isolate was found to be closely related to S. lutetiensis and S. infantarius. Undesirable microorganisms from genera such as Escherichia, Klebsiella, Enterobacter, Acinetobacter and Clostridium were, however, also frequent, or even dominant in Dhanaan samples. Thus, this calls for a change in the Dahnaan manufacturing practice to an improved and safer production system. Starter cultures suitable for Dhanaan production might be developed from the Streptococcus, Weissella, and Lactococcus microorganisms identified in this study. However, further safety evaluation and technological characterization need to be conducted on strains defined by OTU-1, OTU-2, OTU-3, OTU-8, and OTU-35 before they can be used as food grade starter cultures.
Original languageEnglish
Article numberfnz128
JournalFEMS Microbiology Letters
Volume366
Issue number11
Number of pages6
ISSN0378-1097
DOIs
Publication statusPublished - 2019

Keywords

  • Dhanaan
  • Metagenomic analysis
  • OTU
  • fermented camel milk
  • lactic acid bacteria

Cite this

Berhe, Tesfemariam ; Ipsen, Richard ; Seifu, Eyassu ; Kurtu, Mohamed Y ; Fugl, Angelina June Brandt ; Hansen, Egon Bech. / Metagenomic analysis of bacterial community composition in Dhanaan: Ethiopian traditional fermented camel milk. In: FEMS Microbiology Letters. 2019 ; Vol. 366, No. 11.
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abstract = "This study was conducted to evaluate the safety and bacterial profile of Dhanaan (Ethiopian traditional fermented camel milk). The composition of the microbial community in Dhanaan samples was analysed by a metagenomic approach of 16S rRNA gene amplicon sequencing. Metagenomic profiling identified 87 different bacterial microorganisms (OTUs) in six samples analysed. Although the Dhanaan samples contained various lactic acid bacteria (LAB), they also all contained undesirable microorganisms in large proportions. The following LAB genera were identified: Streptococcus, Lactococcus, and Weissella. One Streptococcus species represented by OTU-1 (operational taxonomic unit) was found in all Dhanaan samples and the dominating species in four out of six samples. This common isolate was found to be closely related to S. lutetiensis and S. infantarius. Undesirable microorganisms from genera such as Escherichia, Klebsiella, Enterobacter, Acinetobacter and Clostridium were, however, also frequent, or even dominant in Dhanaan samples. Thus, this calls for a change in the Dahnaan manufacturing practice to an improved and safer production system. Starter cultures suitable for Dhanaan production might be developed from the Streptococcus, Weissella, and Lactococcus microorganisms identified in this study. However, further safety evaluation and technological characterization need to be conducted on strains defined by OTU-1, OTU-2, OTU-3, OTU-8, and OTU-35 before they can be used as food grade starter cultures.",
keywords = "Dhanaan, Metagenomic analysis, OTU, fermented camel milk, lactic acid bacteria",
author = "Tesfemariam Berhe and Richard Ipsen and Eyassu Seifu and Kurtu, {Mohamed Y} and Fugl, {Angelina June Brandt} and Hansen, {Egon Bech}",
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Metagenomic analysis of bacterial community composition in Dhanaan: Ethiopian traditional fermented camel milk. / Berhe, Tesfemariam; Ipsen, Richard; Seifu, Eyassu; Kurtu, Mohamed Y; Fugl, Angelina June Brandt; Hansen, Egon Bech.

In: FEMS Microbiology Letters, Vol. 366, No. 11, fnz128, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Metagenomic analysis of bacterial community composition in Dhanaan: Ethiopian traditional fermented camel milk

AU - Berhe, Tesfemariam

AU - Ipsen, Richard

AU - Seifu, Eyassu

AU - Kurtu, Mohamed Y

AU - Fugl, Angelina June Brandt

AU - Hansen, Egon Bech

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N2 - This study was conducted to evaluate the safety and bacterial profile of Dhanaan (Ethiopian traditional fermented camel milk). The composition of the microbial community in Dhanaan samples was analysed by a metagenomic approach of 16S rRNA gene amplicon sequencing. Metagenomic profiling identified 87 different bacterial microorganisms (OTUs) in six samples analysed. Although the Dhanaan samples contained various lactic acid bacteria (LAB), they also all contained undesirable microorganisms in large proportions. The following LAB genera were identified: Streptococcus, Lactococcus, and Weissella. One Streptococcus species represented by OTU-1 (operational taxonomic unit) was found in all Dhanaan samples and the dominating species in four out of six samples. This common isolate was found to be closely related to S. lutetiensis and S. infantarius. Undesirable microorganisms from genera such as Escherichia, Klebsiella, Enterobacter, Acinetobacter and Clostridium were, however, also frequent, or even dominant in Dhanaan samples. Thus, this calls for a change in the Dahnaan manufacturing practice to an improved and safer production system. Starter cultures suitable for Dhanaan production might be developed from the Streptococcus, Weissella, and Lactococcus microorganisms identified in this study. However, further safety evaluation and technological characterization need to be conducted on strains defined by OTU-1, OTU-2, OTU-3, OTU-8, and OTU-35 before they can be used as food grade starter cultures.

AB - This study was conducted to evaluate the safety and bacterial profile of Dhanaan (Ethiopian traditional fermented camel milk). The composition of the microbial community in Dhanaan samples was analysed by a metagenomic approach of 16S rRNA gene amplicon sequencing. Metagenomic profiling identified 87 different bacterial microorganisms (OTUs) in six samples analysed. Although the Dhanaan samples contained various lactic acid bacteria (LAB), they also all contained undesirable microorganisms in large proportions. The following LAB genera were identified: Streptococcus, Lactococcus, and Weissella. One Streptococcus species represented by OTU-1 (operational taxonomic unit) was found in all Dhanaan samples and the dominating species in four out of six samples. This common isolate was found to be closely related to S. lutetiensis and S. infantarius. Undesirable microorganisms from genera such as Escherichia, Klebsiella, Enterobacter, Acinetobacter and Clostridium were, however, also frequent, or even dominant in Dhanaan samples. Thus, this calls for a change in the Dahnaan manufacturing practice to an improved and safer production system. Starter cultures suitable for Dhanaan production might be developed from the Streptococcus, Weissella, and Lactococcus microorganisms identified in this study. However, further safety evaluation and technological characterization need to be conducted on strains defined by OTU-1, OTU-2, OTU-3, OTU-8, and OTU-35 before they can be used as food grade starter cultures.

KW - Dhanaan

KW - Metagenomic analysis

KW - OTU

KW - fermented camel milk

KW - lactic acid bacteria

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DO - 10.1093/femsle/fnz128

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