Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study

B. Pullinger; H. Profka; J H Ardenkjaer-Larsen; N. N. Kuzma; S. Kadlecek; R. R. Rizi

doi:10.1002/nbm.2777

Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study

B. Pullinger
, H. Profka
, J H Ardenkjaer-Larsen
, N. N. Kuzma
, S. Kadlecek
, R. R. Rizi

GE Healthcare
University of Pennsylvania

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.

Original language	English
Journal	N M R in Biomedicine
Volume	25
Issue number	10
Pages (from-to)	1113-1118
ISSN	0952-3480
DOIs	https://doi.org/10.1002/nbm.2777
Publication status	Published - 2012
Externally published	Yes

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title = "Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study",

abstract = "We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright {\textcopyright} 2012 John Wiley \& Sons, Ltd.",

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doi = "10.1002/nbm.2777",

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T1 - Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study

AU - Pullinger, B.

AU - Profka, H.

AU - Ardenkjaer-Larsen, J H

AU - Kuzma, N. N.

AU - Kadlecek, S.

AU - Rizi, R. R.

PY - 2012

Y1 - 2012

N2 - We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.

AB - We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.

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DO - 10.1002/nbm.2777

M3 - Journal article

C2 - 22311307

SN - 0952-3480

VL - 25

SP - 1113

EP - 1118

JO - N M R in Biomedicine

JF - N M R in Biomedicine

IS - 10

ER -

Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study

Abstract

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