We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p <0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.
Pullinger, B., Profka, H., Ardenkjaer-Larsen, J. H., Kuzma, N. N., Kadlecek, S., & Rizi, R. R. (2012). Metabolism of hyperpolarized [1-(13) C]pyruvate in the isolated perfused rat lung - an ischemia study. N M R in Biomedicine, 25(10), 1113-1118. https://doi.org/10.1002/nbm.2777