This invention relates to the production of fatty acids and particularly to the production of the polyunsaturated fatty acids (PUFAs) arachidonic acid (ARA) and eicosapentaenoic acid (EPA) in genetically engineered fungal cells, in particular, to metabolically engineered Saccharomyces cerevisiae cells with increased content of ARA and EPA. The invention especially involves improvement of the PUFA content in the host organism through various over-expression of e.g. cytochrome b5 and cytochrome b5 reductase involved in fatty acid desaturation, and heterologous expression of cytochrome b5 and cytochrome b5 reductase and expression of heterologous fatty acid synthases.
|Patent number|| WO2008000277|
|Country||International Bureau of the World Intellectual Property Organization (WIPO)|
|Publication status||Published - 2008|