Metabolically engineered cells for the production of pinosylvin

Michael Katz; Jochen Förster; Helga Susana Moreira David; Hans Peter Schmidt; Malin Sendelius; Sara Petersen Bjørn; Thomas Thomasen Durhuus

Metabolically engineered cells for the production of pinosylvin

Michael Katz (Inventor), Jochen Förster (Inventor), Helga Susana Moreira David (Inventor), Hans Peter Schmidt (Inventor), Malin Sendelius (Inventor), Sara Petersen Bjørn (Inventor), Thomas Thomasen Durhuus (Inventor)

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Abstract

A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4- hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

Original language	English
Patent number	WO2007EP57484
Filing date	24/01/2008
Country/Territory	International Bureau of the World Intellectual Property Organization (WIPO)
Priority date	20/07/2007
Priority number	GB20060014442
Publication status	Published - 2008

Bibliographical note

Also published as: ZA200900958 (A); US2014024862 (A1); US2009317881 (A1); US8343739 (B2); NZ574895 (A)

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title = "Metabolically engineered cells for the production of pinosylvin",

abstract = "A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4- hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.",

author = "Michael Katz and Jochen F{\"o}rster and David, {Helga Susana Moreira} and Schmidt, {Hans Peter} and Malin Sendelius and {Petersen Bj{\o}rn}, Sara and Durhuus, {Thomas Thomasen}",

note = "Also published as: ZA200900958 (A); US2014024862 (A1); US2009317881 (A1); US8343739 (B2); NZ574895 (A) ; WO2007EP57484",

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TY - PAT

T1 - Metabolically engineered cells for the production of pinosylvin

AU - Katz, Michael

AU - Förster, Jochen

AU - David, Helga Susana Moreira

AU - Schmidt, Hans Peter

AU - Sendelius, Malin

AU - Petersen Bjørn, Sara

AU - Durhuus, Thomas Thomasen

N1 - Also published as: ZA200900958 (A); US2014024862 (A1); US2009317881 (A1); US8343739 (B2); NZ574895 (A)

PY - 2008

Y1 - 2008

N2 - A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4- hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

AB - A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4- hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

M3 - Patent

M1 - WO2007EP57484

Y2 - 2008/01/24

ER -

Metabolically engineered cells for the production of pinosylvin

Abstract

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