Metabolically engineered cells for the production of pinosylvin

Michael Katz (Inventor), Jochen Förster (Inventor), Helga Susana Moreira David (Inventor), Hans Peter Schmidt (Inventor), Malin Sendelius (Inventor), Sara Petersen Bjørn (Inventor), Thomas Thomasen Durhuus (Inventor)

    Research output: Patent

    106 Downloads (Pure)

    Abstract

    A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4- hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.
    Original languageEnglish
    Patent numberWO2007EP57484
    Filing date24/01/2008
    CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
    Priority date20/07/2007
    Priority numberGB20060014442
    Publication statusPublished - 2008

    Bibliographical note

    Also published as: ZA200900958 (A); US2014024862 (A1); US2009317881 (A1); US8343739 (B2); NZ574895 (A)

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