propionate, and valerate. In addition to these short-chain fatty acids (SCFAs), 13C-labelled lactate, formate, succinate, and ethanol were detected in the colon simulator samples. Metabolite formation and PDX substrate degradation were examined comprehensively over time (24 and 48 hours). Correlation analysis between 13C NMR spectra and gas production confirmed the anaerobic fermentation of PDX to SCFAs. In addition, 16S rRNA gene analysis showed that the
level of Erysipelotrichaceae was influenced by PDX supplementation and Erysipelotrichaceae level were statistically correlated with SCFA’s formation. Overall, our study demonstrates a novel approach to link substrate fermentation and microbial function directly in a simulated colonic environment.
- Gut microbiome
- Dietary fiber
- 13C-labelled metabolites
- 13C NMR