Metabolic fate of 13C-labelled polydextrose and impact on the gut microbiome: A triple-phase study in a colon simulator

Santosh Lamichhane*, Christian C. Yde, Henrik Max Jensen, Wesley Morovic, Ashley A. Hibberd, Arthur C. Ouwehand, Markku T. Saarinen, Sofia D. Forssten, Lars Wiebe, Jørn Marcussen, Kresten Bertelsen, Sebastian Meier, Jette F. Young, Hanne Christine Bertram

*Corresponding author for this work

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Abstract

The present study introduces a novel triple-phase (liquids, solids and gases) approach, which employed uniformly labelled [U-13C] polydextrose (PDX) for the selective profiling of metabolites generated from dietary fiber fermentation in an in vitro colon simulator using human fecal inocula. Employing 13C NMR spectroscopy, [U-13C] PDX metabolism was observed from colonic digest samples. The major 13C-labelled metabolites generated were acetate, butyrate,
propionate, and valerate. In addition to these short-chain fatty acids (SCFAs), 13C-labelled lactate, formate, succinate, and ethanol were detected in the colon simulator samples. Metabolite formation and PDX substrate degradation were examined comprehensively over time (24 and 48 hours). Correlation analysis between 13C NMR spectra and gas production confirmed the anaerobic fermentation of PDX to SCFAs. In addition, 16S rRNA gene analysis showed that the
level of Erysipelotrichaceae was influenced by PDX supplementation and Erysipelotrichaceae level were statistically correlated with SCFA’s formation. Overall, our study demonstrates a novel approach to link substrate fermentation and microbial function directly in a simulated colonic environment.
Original languageEnglish
JournalJournal of Proteome Research
Volume17
Issue number3
Pages (from-to)1041-1053
ISSN1535-3893
DOIs
Publication statusPublished - 2018

Keywords

  • Gut microbiome
  • Dietary fiber
  • 13C-labelled metabolites
  • 13C NMR

Cite this

Lamichhane, S., Yde, C. C., Max Jensen, H., Morovic, W., Hibberd, A. A., Ouwehand, A. C., Saarinen, M. T., Forssten, S. D., Wiebe, L., Marcussen, J., Bertelsen, K., Meier, S., Young, J. F., & Bertram, H. C. (2018). Metabolic fate of 13C-labelled polydextrose and impact on the gut microbiome: A triple-phase study in a colon simulator. Journal of Proteome Research, 17(3), 1041-1053. https://doi.org/10.1021/acs.jproteome.7b00683