TY - JOUR
T1 - Metabolic engineering of yeast for fermentative production of flavonoids
AU - Rodriguez Prado, Edith Angelica
AU - Strucko, Tomas
AU - Stahlhut, Steen Gustav
AU - Kristensen, Mette
AU - Svenssen, Daniel Killerup
AU - Förster, Jochen
AU - Nielsen, Jens
AU - Borodina, Irina
PY - 2017
Y1 - 2017
N2 - Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57±2.66mgL-1 and quercetin 20.38±2.57mgL-1 exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.
AB - Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57±2.66mgL-1 and quercetin 20.38±2.57mgL-1 exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.
KW - Fisetin
KW - Flavonoids
KW - Kaempferol
KW - Resokaempferol
KW - Saccharomyces cerevisiae
U2 - 10.1016/j.biortech.2017.06.043
DO - 10.1016/j.biortech.2017.06.043
M3 - Journal article
C2 - 28634125
SN - 0960-8524
VL - 245
SP - 1645
EP - 1654
JO - Bioresource Technology
JF - Bioresource Technology
IS - Part B
ER -