Metabolic engineering of yeast for fermentative production of flavonoids

Edith Angelica Rodriguez Prado, Tomas Strucko, Steen Gustav Stahlhut, Mette Kristensen, Daniel Killerup Svenssen, Jochen Förster, Jens Nielsen, Irina Borodina

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Abstract

Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57±2.66mgL-1 and quercetin 20.38±2.57mgL-1 exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.
Original languageEnglish
JournalBioresource Technology
Volume245
Issue numberPart B
Pages (from-to)1645-1654
ISSN0960-8524
DOIs
Publication statusPublished - 2017

Keywords

  • Fisetin
  • Flavonoids
  • Kaempferol
  • Resokaempferol
  • Saccharomyces cerevisiae

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