Metabolic control analysis of Aspergillus niger L-arabinose catabolism

M.J.L. de Groot, Wai Prathumpai, J. Visser, G.J.G. Ruijter

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A mathematical model of the L-arabinose/D-xylose catabolic pathway of Aspergillus niger was constructed based on the kinetic properties of the enzymes. For this purpose L-arabinose reductase, L-arabitol dehydrogenase and D-xylose reductase were purified using dye-affinity chromatography, and their kinetic properties were characterized. For the other enzymes of the pathway the kinetic data were available from the literature. The metabolic model was used to analyze flux and metabolite concentration control of the L-arabinose catabolic pathway. The model demonstrated that flux control does not reside at the enzyme following the intermediate with the highest concentration, L-arabitol, but is distributed over the first three steps in the pathway, preceding and following L-arabitol. Flux control appeared to be strongly dependent on the intracellular L-arabinose concentration. At 5 mM intracellular L-arabinose, a level that resulted in realistic intermediate concentrations in the model, flux control coefficients for L-arabinose reductase, L-arabitol dehydrogenase and L-xylulose reductase were 0.68, 0.17 and 0.14, respectively. The analysis can be used as a guide to identify targets for metabolic engineering aiming at either flux or metabolite level optimization of the L-arabinose catabolic pathway of A. niger. Faster L-arabinose utilization may enhance utilization of readily available organic waste containing hemicelluloses to be converted into industrially interesting metabolites or valuable enzymes or proteins.
    Original languageEnglish
    JournalBiotechnology Progress
    Volume21
    Issue number6
    Pages (from-to)1610-1616
    ISSN8756-7938
    Publication statusPublished - 2005

    Cite this

    de Groot, M. J. L., Prathumpai, W., Visser, J., & Ruijter, G. J. G. (2005). Metabolic control analysis of Aspergillus niger L-arabinose catabolism. Biotechnology Progress, 21(6), 1610-1616.
    de Groot, M.J.L. ; Prathumpai, Wai ; Visser, J. ; Ruijter, G.J.G. / Metabolic control analysis of Aspergillus niger L-arabinose catabolism. In: Biotechnology Progress. 2005 ; Vol. 21, No. 6. pp. 1610-1616.
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    title = "Metabolic control analysis of Aspergillus niger L-arabinose catabolism",
    abstract = "A mathematical model of the L-arabinose/D-xylose catabolic pathway of Aspergillus niger was constructed based on the kinetic properties of the enzymes. For this purpose L-arabinose reductase, L-arabitol dehydrogenase and D-xylose reductase were purified using dye-affinity chromatography, and their kinetic properties were characterized. For the other enzymes of the pathway the kinetic data were available from the literature. The metabolic model was used to analyze flux and metabolite concentration control of the L-arabinose catabolic pathway. The model demonstrated that flux control does not reside at the enzyme following the intermediate with the highest concentration, L-arabitol, but is distributed over the first three steps in the pathway, preceding and following L-arabitol. Flux control appeared to be strongly dependent on the intracellular L-arabinose concentration. At 5 mM intracellular L-arabinose, a level that resulted in realistic intermediate concentrations in the model, flux control coefficients for L-arabinose reductase, L-arabitol dehydrogenase and L-xylulose reductase were 0.68, 0.17 and 0.14, respectively. The analysis can be used as a guide to identify targets for metabolic engineering aiming at either flux or metabolite level optimization of the L-arabinose catabolic pathway of A. niger. Faster L-arabinose utilization may enhance utilization of readily available organic waste containing hemicelluloses to be converted into industrially interesting metabolites or valuable enzymes or proteins.",
    author = "{de Groot}, M.J.L. and Wai Prathumpai and J. Visser and G.J.G. Ruijter",
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    de Groot, MJL, Prathumpai, W, Visser, J & Ruijter, GJG 2005, 'Metabolic control analysis of Aspergillus niger L-arabinose catabolism', Biotechnology Progress, vol. 21, no. 6, pp. 1610-1616.

    Metabolic control analysis of Aspergillus niger L-arabinose catabolism. / de Groot, M.J.L.; Prathumpai, Wai; Visser, J.; Ruijter, G.J.G.

    In: Biotechnology Progress, Vol. 21, No. 6, 2005, p. 1610-1616.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Metabolic control analysis of Aspergillus niger L-arabinose catabolism

    AU - de Groot, M.J.L.

    AU - Prathumpai, Wai

    AU - Visser, J.

    AU - Ruijter, G.J.G.

    PY - 2005

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    N2 - A mathematical model of the L-arabinose/D-xylose catabolic pathway of Aspergillus niger was constructed based on the kinetic properties of the enzymes. For this purpose L-arabinose reductase, L-arabitol dehydrogenase and D-xylose reductase were purified using dye-affinity chromatography, and their kinetic properties were characterized. For the other enzymes of the pathway the kinetic data were available from the literature. The metabolic model was used to analyze flux and metabolite concentration control of the L-arabinose catabolic pathway. The model demonstrated that flux control does not reside at the enzyme following the intermediate with the highest concentration, L-arabitol, but is distributed over the first three steps in the pathway, preceding and following L-arabitol. Flux control appeared to be strongly dependent on the intracellular L-arabinose concentration. At 5 mM intracellular L-arabinose, a level that resulted in realistic intermediate concentrations in the model, flux control coefficients for L-arabinose reductase, L-arabitol dehydrogenase and L-xylulose reductase were 0.68, 0.17 and 0.14, respectively. The analysis can be used as a guide to identify targets for metabolic engineering aiming at either flux or metabolite level optimization of the L-arabinose catabolic pathway of A. niger. Faster L-arabinose utilization may enhance utilization of readily available organic waste containing hemicelluloses to be converted into industrially interesting metabolites or valuable enzymes or proteins.

    AB - A mathematical model of the L-arabinose/D-xylose catabolic pathway of Aspergillus niger was constructed based on the kinetic properties of the enzymes. For this purpose L-arabinose reductase, L-arabitol dehydrogenase and D-xylose reductase were purified using dye-affinity chromatography, and their kinetic properties were characterized. For the other enzymes of the pathway the kinetic data were available from the literature. The metabolic model was used to analyze flux and metabolite concentration control of the L-arabinose catabolic pathway. The model demonstrated that flux control does not reside at the enzyme following the intermediate with the highest concentration, L-arabitol, but is distributed over the first three steps in the pathway, preceding and following L-arabitol. Flux control appeared to be strongly dependent on the intracellular L-arabinose concentration. At 5 mM intracellular L-arabinose, a level that resulted in realistic intermediate concentrations in the model, flux control coefficients for L-arabinose reductase, L-arabitol dehydrogenase and L-xylulose reductase were 0.68, 0.17 and 0.14, respectively. The analysis can be used as a guide to identify targets for metabolic engineering aiming at either flux or metabolite level optimization of the L-arabinose catabolic pathway of A. niger. Faster L-arabinose utilization may enhance utilization of readily available organic waste containing hemicelluloses to be converted into industrially interesting metabolites or valuable enzymes or proteins.

    M3 - Journal article

    VL - 21

    SP - 1610

    EP - 1616

    JO - Biotechnology Progress

    JF - Biotechnology Progress

    SN - 8756-7938

    IS - 6

    ER -

    de Groot MJL, Prathumpai W, Visser J, Ruijter GJG. Metabolic control analysis of Aspergillus niger L-arabinose catabolism. Biotechnology Progress. 2005;21(6):1610-1616.