Metabolic and regulatory insights from the experimental Horizontal Gene Transfer of the aurofusarin and bikaverin gene clusters to Aspergillus nidulans

Elise de Reus, Mikkel Rank Nielsen, Rasmus John Normand Frandsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

35 Downloads (Pure)

Abstract

We staged the transfer of the aurofusarin and bikaverin biosynthetic gene clusters (BGCs) to Aspergillus nidulans with the aim of gaining functional insights into dynamics immediately following a horizontal gene transfer (HGT) event. While the introduction of both BGCs resulted in the production of detectable pathway metabolites in A. nidulans, the transferred aurofusarin BGC formed dimeric shunt products instead of aurofusarin. This was linked to low transcription of the cluster activator and insufficient activity of tailoring enzymes, demonstrating how a shift of the pathway bottleneck after HGT can result in metabolic innovation. The transferred bikaverin BGC readily produced bikaverin, providing a model system for studying the conservation of regulatory responses to environmental cues. Conserved PacC-mediated pH regulation of the bikaverin BGC was observed between original host Fusarium fujikuroi and A. nidulans. Contrary to strong nitrogen responses described in other hosts, the BGC appeared unresponsive to environmental nitrogen in A. nidulans. While F. fujikuroi and A. nidulans both form chlamydospore-like structures when exposed to ralsolamycin, specific induction of the bikaverin BGC was not observed in A. nidulans. We propose that the presence of compatible cis-regulatory elements in BGCs facilitates regulatory conservation after transfer, without which the chromosomal context would dictate expression.
Original languageEnglish
JournalMolecular Microbiology
Volume112
Issue number6
Pages (from-to)1684-1700
Number of pages17
ISSN0950-382X
DOIs
Publication statusPublished - 2019

Keywords

  • Ascomycota
  • Gene Expression Regulation
  • Gene clusters
  • Horizontal gene transfer
  • Secondary metabolism

Fingerprint Dive into the research topics of 'Metabolic and regulatory insights from the experimental Horizontal Gene Transfer of the aurofusarin and bikaverin gene clusters to Aspergillus nidulans'. Together they form a unique fingerprint.

Cite this