Membrane separation of enzyme-converted biomass compounds: Recovery of xylose and production of gluconic acid as a value-added product

Sofie Thage Morthensen, Birgitte Zeuner, Anne S. Meyer, Henning Jørgensen, Manuel Pinelo*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The purpose of the present study was to assess the efficiency of enzyme-assisted nanofiltration for separation of xylose from glucose present in genuine biorefinery liquors obtained from hydrothermal pretreatment of wheat straw, corn stover and Miscanthus stalks. Glucose oxidase and catalase were used to convert the glucose contained in the liquors into gluconic acid, so xylose could be more easily recovered in the subsequent nanofiltration. Subjecting the biomass liquors to dilute acid treatment and centrifugation before the enzymatic reaction and filtration led to maximum biocatalytic performance of the membrane bioreactor (neglectable fouling and no enzyme activity loss) during five consecutive reaction-filtration cycles. The best separation factor of gluconic acid over xylose in the subsequent nanofiltration was 2.7, 2.5 and 2.2 for wheat straw, corn stover and Miscanthus stalks, respectively. All represented a significant improvement compared to the benchmark separation of xylose and glucose, in which case the separation factor was only 1.4. However, the higher ionic strength of the biomass liquors compared to the pure model solution probably led to a less negative zeta potential of the nanofiltration membrane, which significantly reduced the xylose purification performance as compared to the model system, for which the separation factor was 34.
Original languageEnglish
JournalSeparation and Purification Technology
Volume194
Pages (from-to)73-80
ISSN1383-5866
DOIs
Publication statusPublished - 2018

Keywords

  • Membrane bioreactor
  • Biomass liquor
  • Glucose oxidase/catalase
  • Sugars
  • Low-pressure nanofiltration

Cite this

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title = "Membrane separation of enzyme-converted biomass compounds: Recovery of xylose and production of gluconic acid as a value-added product",
abstract = "The purpose of the present study was to assess the efficiency of enzyme-assisted nanofiltration for separation of xylose from glucose present in genuine biorefinery liquors obtained from hydrothermal pretreatment of wheat straw, corn stover and Miscanthus stalks. Glucose oxidase and catalase were used to convert the glucose contained in the liquors into gluconic acid, so xylose could be more easily recovered in the subsequent nanofiltration. Subjecting the biomass liquors to dilute acid treatment and centrifugation before the enzymatic reaction and filtration led to maximum biocatalytic performance of the membrane bioreactor (neglectable fouling and no enzyme activity loss) during five consecutive reaction-filtration cycles. The best separation factor of gluconic acid over xylose in the subsequent nanofiltration was 2.7, 2.5 and 2.2 for wheat straw, corn stover and Miscanthus stalks, respectively. All represented a significant improvement compared to the benchmark separation of xylose and glucose, in which case the separation factor was only 1.4. However, the higher ionic strength of the biomass liquors compared to the pure model solution probably led to a less negative zeta potential of the nanofiltration membrane, which significantly reduced the xylose purification performance as compared to the model system, for which the separation factor was 34.",
keywords = "Membrane bioreactor, Biomass liquor, Glucose oxidase/catalase, Sugars, Low-pressure nanofiltration",
author = "Morthensen, {Sofie Thage} and Birgitte Zeuner and Meyer, {Anne S.} and Henning J{\o}rgensen and Manuel Pinelo",
year = "2018",
doi = "10.1016/j.seppur.2017.11.031",
language = "English",
volume = "194",
pages = "73--80",
journal = "Separation and Purification Technology",
issn = "1383-5866",
publisher = "Pergamon Press",

}

Membrane separation of enzyme-converted biomass compounds: Recovery of xylose and production of gluconic acid as a value-added product. / Morthensen, Sofie Thage; Zeuner, Birgitte; Meyer, Anne S.; Jørgensen, Henning; Pinelo, Manuel.

In: Separation and Purification Technology, Vol. 194, 2018, p. 73-80.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Membrane separation of enzyme-converted biomass compounds: Recovery of xylose and production of gluconic acid as a value-added product

AU - Morthensen, Sofie Thage

AU - Zeuner, Birgitte

AU - Meyer, Anne S.

AU - Jørgensen, Henning

AU - Pinelo, Manuel

PY - 2018

Y1 - 2018

N2 - The purpose of the present study was to assess the efficiency of enzyme-assisted nanofiltration for separation of xylose from glucose present in genuine biorefinery liquors obtained from hydrothermal pretreatment of wheat straw, corn stover and Miscanthus stalks. Glucose oxidase and catalase were used to convert the glucose contained in the liquors into gluconic acid, so xylose could be more easily recovered in the subsequent nanofiltration. Subjecting the biomass liquors to dilute acid treatment and centrifugation before the enzymatic reaction and filtration led to maximum biocatalytic performance of the membrane bioreactor (neglectable fouling and no enzyme activity loss) during five consecutive reaction-filtration cycles. The best separation factor of gluconic acid over xylose in the subsequent nanofiltration was 2.7, 2.5 and 2.2 for wheat straw, corn stover and Miscanthus stalks, respectively. All represented a significant improvement compared to the benchmark separation of xylose and glucose, in which case the separation factor was only 1.4. However, the higher ionic strength of the biomass liquors compared to the pure model solution probably led to a less negative zeta potential of the nanofiltration membrane, which significantly reduced the xylose purification performance as compared to the model system, for which the separation factor was 34.

AB - The purpose of the present study was to assess the efficiency of enzyme-assisted nanofiltration for separation of xylose from glucose present in genuine biorefinery liquors obtained from hydrothermal pretreatment of wheat straw, corn stover and Miscanthus stalks. Glucose oxidase and catalase were used to convert the glucose contained in the liquors into gluconic acid, so xylose could be more easily recovered in the subsequent nanofiltration. Subjecting the biomass liquors to dilute acid treatment and centrifugation before the enzymatic reaction and filtration led to maximum biocatalytic performance of the membrane bioreactor (neglectable fouling and no enzyme activity loss) during five consecutive reaction-filtration cycles. The best separation factor of gluconic acid over xylose in the subsequent nanofiltration was 2.7, 2.5 and 2.2 for wheat straw, corn stover and Miscanthus stalks, respectively. All represented a significant improvement compared to the benchmark separation of xylose and glucose, in which case the separation factor was only 1.4. However, the higher ionic strength of the biomass liquors compared to the pure model solution probably led to a less negative zeta potential of the nanofiltration membrane, which significantly reduced the xylose purification performance as compared to the model system, for which the separation factor was 34.

KW - Membrane bioreactor

KW - Biomass liquor

KW - Glucose oxidase/catalase

KW - Sugars

KW - Low-pressure nanofiltration

U2 - 10.1016/j.seppur.2017.11.031

DO - 10.1016/j.seppur.2017.11.031

M3 - Journal article

VL - 194

SP - 73

EP - 80

JO - Separation and Purification Technology

JF - Separation and Purification Technology

SN - 1383-5866

ER -