Mediatorless Carbohydrate/Oxygen Biofuel Cells with Improved Cellobiose Dehydrogenase Based Bioanode

Vida Krikstolaityte, P. Lamberg, M. D. Toscano, M. Silow, O. Eicher‐Lorka, A. Ramanavicius, G. Niaura, L. Abariute, T. Ruzgas, S. Shleev

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Direct electron transfer (DET) between cellobiose dehydrogenase from Humicola insolens ascomycete (HiCDH) and gold nanoparticles (AuNPs) was achieved by modifying AuNPs with a novel, positively charged thiol N‐(6‐mercapto)hexylpyridinium (MHP). The DET enabled the use of the HiCDH enzyme as an anodic biocatalyst in the design of a mediatorless carbohydrate/oxygen enzymatic fuel cell (EFC). A biocathode of the EFC was based on bilirubin oxidase from Myrothecium verrucaria (MvBOx) directly immobilised on the surface of AuNPs. The following parameters of the EFC based on Au/AuNP/MHP/HiCDH bioanode and Au/AuNP/MvBOx biocathode were obtained in quiescent air saturated PBS, pH 7.4, containing: (i) 5 mM glucose‐open‐circuit voltage (OCV) of 0.65 ± 0.011 V and the maximal power density of 4.77 ± 1.34 μW cm−2 at operating voltage of 0.50 V; or (ii) 10 mM lactose‐OCV of 0.67 ± 0.006 V and the maximal power density of 8.64 ± 1.91 μW cm−2 at operating voltage of 0.50 V. The half‐life operation times of the EFC were estimated to be at least 13 and 44 h in air saturated PBS containing 5 mM glucose and 10 mM lactose, respectively. Among advantages of HiCDH/MvBOx FCs are (i) simplified construction, (ii) relatively high power output with glucose as biofuel, and (iii) the absence of the inhibition of the HiCDH based bioanode by lactose, when compared with the best previously reported CDH based bioanode.
Original languageEnglish
JournalFuel Cells
Volume14
Issue number6
Pages (from-to)792-800
Number of pages9
ISSN1615-6846
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Cellobiose Dehydrogenase
  • Bilirubin Oxidase
  • Direct Electron Transfer
  • Enzymatic Fuel Cell
  • Gold Nanoparticle
  • N‐(6‐mercapto)hexylpyridinium

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