Mechanism of post-segregational kilting by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killingof plasmid-free cells

K. Gerdes; T. Thisted; Jan Martinussen

doi:10.1111/j.1365-2958.1990.tb02029.x

Mechanism of post-segregational kilting by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killingof plasmid-free cells

K. Gerdes, T. Thisted, Jan Martinussen

University of Southern Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The hok/sok system of plasmid R1, which mediates plasmid stabilization via killing of plasmid-free segregants, encodes two genes: hok and sok. The hok gene product is a potent cell-killing protein. The expression of hok is regulated post-transcriptionally by the sok gene-encoded repressor, an antisense RNA complementary to the hok mRNA leader region. We show here that the hok mRNA is very stable, while the sok RNA decays rapidly. We also observe a new hok mRNA species which is 70 nucleotides shorter in the 3'-end than the full-length hok transcript. The appearance of the truncated hok mRNA was found to be regulated by the sok antisense RNA. Furthermore, the presence of the truncated hok mRNA was found to be correlated with efficient expression of the Hok protein. On the basis of these findings, we propose an extended model in order to explain the killing of plasmid-free segregants by the hok/sok system.

Original language	English
Journal	Molecular Microbiology
Volume	4
Issue number	11
Pages (from-to)	1807-1818
Number of pages	12
ISSN	0950-382X
DOIs	https://doi.org/10.1111/j.1365-2958.1990.tb02029.x
Publication status	Published - 1990
Externally published	Yes

Keywords

Base Sequence
Escherichia coli
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genes, Regulator
Molecular Sequence Data
Plasmids
RNA, Antisense
RNA, Bacterial
RNA, Messenger
Transcription, Genetic
ESCHERICHIA-COLI REPRESSOR MESSENGER STABILITY TRUNCATED TRANSCRIPT NUCLEOTIDE SEQUENCE MOLECULAR SEQUENCE DATA
Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702]
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10064, Biochemistry studies - Proteins, peptides and amino acids
10300, Replication, transcription, translation
10506, Biophysics - Molecular properties and macromolecules
13012, Metabolism - Proteins, peptides and amino acids
13014, Metabolism - Nucleic acids, purines and pyrimidines
31000, Physiology and biochemistry of bacteria
31500, Genetics of bacteria and viruses
Biochemistry and Molecular Biophysics
Genetics
Metabolism
Molecular Genetics
Physiology

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10.1111/j.1365-2958.1990.tb02029.x

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title = "Mechanism of post-segregational kilting by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killingof plasmid-free cells",

abstract = "The hok/sok system of plasmid R1, which mediates plasmid stabilization via killing of plasmid-free segregants, encodes two genes: hok and sok. The hok gene product is a potent cell-killing protein. The expression of hok is regulated post-transcriptionally by the sok gene-encoded repressor, an antisense RNA complementary to the hok mRNA leader region. We show here that the hok mRNA is very stable, while the sok RNA decays rapidly. We also observe a new hok mRNA species which is 70 nucleotides shorter in the 3'-end than the full-length hok transcript. The appearance of the truncated hok mRNA was found to be regulated by the sok antisense RNA. Furthermore, the presence of the truncated hok mRNA was found to be correlated with efficient expression of the Hok protein. On the basis of these findings, we propose an extended model in order to explain the killing of plasmid-free segregants by the hok/sok system.",

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author = "K. Gerdes and T. Thisted and Jan Martinussen",

year = "1990",

doi = "10.1111/j.1365-2958.1990.tb02029.x",

language = "English",

volume = "4",

pages = "1807--1818",

journal = "Molecular Microbiology",

issn = "0950-382X",

publisher = "Wiley-Blackwell",

number = "11",

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Mechanism of post-segregational kilting by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killingof plasmid-free cells. / Gerdes, K.; Thisted, T.; Martinussen, Jan.
In: Molecular Microbiology, Vol. 4, No. 11, 1990, p. 1807-1818.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Mechanism of post-segregational kilting by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killingof plasmid-free cells

AU - Gerdes, K.

AU - Thisted, T.

AU - Martinussen, Jan

PY - 1990

Y1 - 1990

N2 - The hok/sok system of plasmid R1, which mediates plasmid stabilization via killing of plasmid-free segregants, encodes two genes: hok and sok. The hok gene product is a potent cell-killing protein. The expression of hok is regulated post-transcriptionally by the sok gene-encoded repressor, an antisense RNA complementary to the hok mRNA leader region. We show here that the hok mRNA is very stable, while the sok RNA decays rapidly. We also observe a new hok mRNA species which is 70 nucleotides shorter in the 3'-end than the full-length hok transcript. The appearance of the truncated hok mRNA was found to be regulated by the sok antisense RNA. Furthermore, the presence of the truncated hok mRNA was found to be correlated with efficient expression of the Hok protein. On the basis of these findings, we propose an extended model in order to explain the killing of plasmid-free segregants by the hok/sok system.

AB - The hok/sok system of plasmid R1, which mediates plasmid stabilization via killing of plasmid-free segregants, encodes two genes: hok and sok. The hok gene product is a potent cell-killing protein. The expression of hok is regulated post-transcriptionally by the sok gene-encoded repressor, an antisense RNA complementary to the hok mRNA leader region. We show here that the hok mRNA is very stable, while the sok RNA decays rapidly. We also observe a new hok mRNA species which is 70 nucleotides shorter in the 3'-end than the full-length hok transcript. The appearance of the truncated hok mRNA was found to be regulated by the sok antisense RNA. Furthermore, the presence of the truncated hok mRNA was found to be correlated with efficient expression of the Hok protein. On the basis of these findings, we propose an extended model in order to explain the killing of plasmid-free segregants by the hok/sok system.

KW - Base Sequence

KW - Escherichia coli

KW - Gene Expression Regulation, Bacterial

KW - Genes, Bacterial

KW - Genes, Regulator

KW - Molecular Sequence Data

KW - Plasmids

KW - RNA, Antisense

KW - RNA, Bacterial

KW - RNA, Messenger

KW - Transcription, Genetic

KW - ESCHERICHIA-COLI REPRESSOR MESSENGER STABILITY TRUNCATED TRANSCRIPT NUCLEOTIDE SEQUENCE MOLECULAR SEQUENCE DATA

KW - Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702]

KW - 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines

KW - 10064, Biochemistry studies - Proteins, peptides and amino acids

KW - 10300, Replication, transcription, translation

KW - 10506, Biophysics - Molecular properties and macromolecules

KW - 13012, Metabolism - Proteins, peptides and amino acids

KW - 13014, Metabolism - Nucleic acids, purines and pyrimidines

KW - 31000, Physiology and biochemistry of bacteria

KW - 31500, Genetics of bacteria and viruses

KW - Biochemistry and Molecular Biophysics

KW - Genetics

KW - Metabolism

KW - Molecular Genetics

KW - Physiology

U2 - 10.1111/j.1365-2958.1990.tb02029.x

DO - 10.1111/j.1365-2958.1990.tb02029.x

M3 - Journal article

SN - 0950-382X

VL - 4

SP - 1807

EP - 1818

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 11

ER -

Mechanism of post-segregational kilting by the hok/sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killingof plasmid-free cells

Abstract

Keywords

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