Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane

Rosanna La Rocca, Rossana Tallerico, Almosawy Talib Hassan, Gobind Das, Lakshmikanth Tadepally, Marco Matteucci, Carlo Liberale, Maria Mesuraca, Domenica Scumaci, Francesco Gentile, Gheorghe Cojoc, Gerardo Perozziello, Antonio Ammendolia, Adriana Gallo, Klas Kärre, Giovanni Cuda, Patrizio Candeloro, Enzo Di Fabrizio, Ennio Carbone

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    Abstract

    In our body, cells are continuously exposed to physical forces that can regulate different cell functions such as cell proliferation, differentiation and death. In this work, we employed two different strategies to mechanically stress cancer cells. The cancer and healthy cell populations were treated either with mechanical stress delivered by a micropump (fabricated by deep X-ray nanolithography) or by ultrasound wave stimuli. A specific down-regulation of Major Histocompatibility Complex (MHC) class I molecules expression on cancer cell membrane compared to different kinds of healthy cells (fibroblasts, macrophages, dendritic and lymphocyte cells) was observed, stimulating the cells with forces in the range of nano-newton, and pressures between 1 and 10 bar (1 bar5100.000 Pascal), depending on the devices used. Moreover, Raman spectroscopy analysis, after mechanical treatment, in the range between 700–1800 cm-1, indicated a relative concentration variation of MHC class I. PCA analysis was also performed to distinguish control and stressed cells within different cell lines. These mechanical induced phenotypic changes increase the tumor immunogenicity, as revealed by the related increased susceptibility to Natural Killer (NK) cells cytotoxic recognition.
    Original languageEnglish
    JournalP L o S One
    Number of pages20
    ISSN1932-6203
    DOIs
    Publication statusPublished - 2014

    Bibliographical note

    ©2014 La Rocca et al. This is an open-access article distributed under the terms of
    the Creative Commons Attribution License

    Cite this

    La Rocca, R., Tallerico, R., Hassan, A. T., Das, G., Tadepally, L., Matteucci, M., Liberale, C., Mesuraca, M., Scumaci, D., Gentile, F., Cojoc, G., Perozziello, G., Ammendolia, A., Gallo, A., Kärre, K., Cuda, G., Candeloro, P., Di Fabrizio, E., & Carbone, E. (2014). Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane. P L o S One. https://doi.org/10.1371/journal.pone.0111758