Mechanical properties of electrospun PCL scaffold under in vitro and accelerated degradation conditions

Alexandra Liv Vest Løvdal, Jakob Vange, Lene Feldskov Nielsen, Kristoffer Almdal

    Research output: Contribution to journalJournal articleResearchpeer-review

    1 Downloads (Pure)

    Abstract

    Within recent years, researchers have looked into using polycaprolactone (PCL) as a synthetic biodegradable scaffold for tissue engineering purposes. This study investigated the mechanical properties of an electrospun PCL, while being exposed to physiological fluids at 37C (in vitro conditions) with and without the influence of cell in-growth. The molecular weight and mechanical properties were monitored during the degradation. Incubation in physiological fluids for 3–16 weeks showed an improvement in mechanical properties and no reduction in molecular weight. It was also shown that cells did not deteriorate the mechanical properties of PCL after 16 weeks. The viability of the cells decreased over time, however, without influencing the mechanical properties of the scaffold. A relation between
    reduction in molecular weight and the mechanical properties of electrospun PCL was seen between 2–29 days in buffer (pH 12). The accelerated study showed a linear decrease in both elastic modulus and yield stress as a function of degradation time.
    Original languageEnglish
    JournalBiomedical Engineering: Applications, Basis and Communications
    Volume26
    Issue number3
    Pages (from-to)1450043
    ISSN1016-2372
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Polycaprolactone (PCL)
    • Electrospinning
    • In vitro degradation
    • Mechanical properties
    • In vitro
    • Fibroblasts

    Fingerprint Dive into the research topics of 'Mechanical properties of electrospun PCL scaffold under in vitro and accelerated degradation conditions'. Together they form a unique fingerprint.

    Cite this