Measuring the acoustophoretic contrast factor of living cells in microchannels

P. Augustsson, Rune Barnkob, C. Grenvall, T. Deierborg, P. Brundin, Henrik Bruus, T. Laurell

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    We report a new method, which allows for accurate measurement of the acostophoretic contrast factor Φ of different cell types, an acousto-physical parameter of fundamental importance in microchip acoustophoresis. As a test case the Φ factor is measured for undifferentiated and four-days differentiated cells from a human embryonic ventral mesencephalic cell line. The measured cell Φ factors are distributed around 0.04 and 0.07 for the two cell types, respectively. Despite a close acoustic similarity, the two cell populations are shown to be separable by acoustophoresis.
    Original languageEnglish
    Title of host publicationProceedings of the 14. International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Publication date2010
    Pages1337-1339
    ISBN (Print)978-0-9798064-3-8
    Publication statusPublished - 2010
    Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Groningen, Netherlands
    Duration: 3 Oct 20107 Oct 2010
    Conference number: 14

    Conference

    Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Number14
    CountryNetherlands
    CityGroningen
    Period03/10/201007/10/2010

    Keywords

    • Ultrasound Resonances
    • Micro-PIV
    • Acoustophoresis
    • Cell Handling

    Cite this

    Augustsson, P., Barnkob, R., Grenvall, C., Deierborg, T., Brundin, P., Bruus, H., & Laurell, T. (2010). Measuring the acoustophoretic contrast factor of living cells in microchannels. In Proceedings of the 14. International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1337-1339) http://www.microtas10.org/