Measurement of Flow Velocity and Inference of Liquid Viscosity in a Microfluidic Channel by Fluorescence Photobleaching

Nick J. Carroll, Kaare Hartvig Jensen, Shima Parsa, N. Michele Holbrook, David A. Weitz

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We present a simple, noninvasive method for simultaneous measurement of flow velocity and inference of liquid viscosity in a microfluidic channel. We track the dynamics of a sharp front of photobleached fluorescent dye using a confocal microscope and measure the intensity at a single point downstream of the initial front position. We fit an exact solution of the advection diffusion equation to the fluorescence intensity recovery curve to determine the average flow velocity and the diffusion coefficient of the tracer dye. The dye diffusivity is correlated to solute concentration to infer theological properties of the liquid. This technique provides a simple method for simultaneous elucidation of flow velocity and liquid viscosity in microchannels.
Original languageEnglish
JournalLangmuir
Volume30
Issue number16
Pages (from-to)4868-4874
Number of pages7
ISSN0743-7463
DOIs
Publication statusPublished - 2014

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