Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR

Kirstine Klitgaard Schou; Tim Kåre Jensen; Øystein Angen; Mette Boye

doi:10.1016/j.mimet.2006.12.003

Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR

Kirstine Klitgaard Schou, Tim Kåre Jensen, Øystein Angen, Mette Boye

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.

Original language	English
Journal	Journal of Microbiological Methods
Volume	69
Issue number	2
Pages (from-to)	414-416
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2006.12.003
Publication status	Published - 2007

Keywords

qRT-PCR
Actinobacillus pleurapneumoniae
laser capture microdissection
in vivo gene expression analysis

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10.1016/j.mimet.2006.12.003

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title = "Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR",

abstract = "Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.",

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year = "2007",

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AU - Schou, Kirstine Klitgaard

AU - Jensen, Tim Kåre

AU - Angen, Øystein

AU - Boye, Mette

PY - 2007

Y1 - 2007

N2 - Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.

AB - Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.

KW - qRT-PCR

KW - Actinobacillus pleurapneumoniae

KW - laser capture microdissection

KW - in vivo gene expression analysis

U2 - 10.1016/j.mimet.2006.12.003

DO - 10.1016/j.mimet.2006.12.003

M3 - Journal article

C2 - 17250913

SN - 0167-7012

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SP - 414

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR

Abstract

Keywords

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