Mass Spectrometry of Underivatized 15-Hydroxyeicosatetraenoic Acid and 15-Hydroxyeicosapentaenoic Acid

K. Fogh, K. Kragballe, E. Larsen, H. Egsgaard, V.K. Shukla

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A direct mass spectrometric method for 15‐hydroxy metabolites of arachidonic acid and of eicosapentaenoic acid is described. 15‐Hydroxyeicosanoids have the capacity to inhibit the formation of leukotrienes, potent mediators of inflammation formed by the 5‐lipoxygenase pathway of arachidonic acid metabolism and may therefore have anti‐inflammatory properties. 15‐Hydroxyeicosatetraenoic acid (15‐HETE) and 15‐hydroxyeicosapentaenoic acid (15‐HEPE) were synthesized using soybean lipoxygenase and arachidonic acid and eicosapentaenoic acid as substrates. These hydroxy fatty acids were then purified by reversed‐phase high‐performance liquid chromatography. This modified procedure provides rapid synthesis of multimilligram quantities of 15‐hydroxyeicosanoids for in vitro and in vivo studies. Electron impact mass spectra of 15‐HETE and 15‐HEPE could be obtained directly without derivatizations.
    Original languageEnglish
    JournalJournal of Mass Spectrometry
    Volume17
    Issue number6
    Pages (from-to)459-461
    ISSN1052-9306
    DOIs
    Publication statusPublished - 1988

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