Mammalian pre-mRNA 3 ' End Processing Factor CF I(m)68 Functions in mRNA Export

Marc-David Ruepp, Chiara Aringhieri, Silvia Vivarelli, Stefano Cardinale, Simona Paro, Daniel Schuemperli, Silvia M. L. Barabino

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3' end formation and export, it is not known how these two processes are mechanistically connected. Here, we show that a subunit of the mammalian pre-mRNA 3' end processing complex, CF I(m)68, stimulates mRNA export. CF I(m)68 shuttles between the nucleus and the cytoplasm in a transcription-dependent manner and interacts with the mRNA export receptor NXF1/TAP. Consistent with the idea that CF I(m)68 may act as a novel adaptor for NXF1/TAP, we show that CF I(m)68 promotes the export of a reporter mRNA as well as of endogenous mRNAs, whereas silencing by RNAi results in the accumulation of mRNAs in the nucleus. Moreover, CF I(m)68 associates with 80S ribosomes but not polysomes, suggesting that it is part of the mRNP that is remodeled in the cytoplasm during the initial stages of translation. These results reveal a novel function for the pre-mRNA 3' end processing factor CF I(m)68 in mRNA export.
Original languageEnglish
JournalMolecular Biology of the Cell
Volume20
Issue number24
Pages (from-to)5211-5223
Number of pages13
ISSN1059-1524
DOIs
Publication statusPublished - 2009
Externally publishedYes

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