Major histocompatibility complex class I binding predictions as a tool in epitope discovery

Claus Lundegaard, Ole Lund, Søren Buus, Morten Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Over the last decade, in silico models of the major histocompatibility complex (MHC) class I pathway have developed significantly. Before, peptide binding could only be reliably modelled for a few major human or mouse histocompatibility molecules; now, high-accuracy predictions are available for any human leucocyte antigen (HLA) -A or -B molecule with known protein sequence. Furthermore, peptide binding to MHC molecules from several non-human primates, mouse strains and other mammals can now be predicted. In this review, a number of different prediction methods are briefly explained, highlighting the most useful and historically important. Selected case stories, where these 'reverse immunology' systems have been used in actual epitope discovery, are briefly reviewed. We conclude that this new generation of epitope discovery systems has become a highly efficient tool for epitope discovery, and recommend that the less accurate prediction systems of the past be abandoned, as these are obsolete.
    Original languageEnglish
    JournalImmunology
    Volume130
    Issue number3
    Pages (from-to)309-318
    ISSN0019-2805
    DOIs
    Publication statusPublished - 2010

    Keywords

    • epitope prediction
    • cytotoxic T lymphocytes
    • human leucocyte antigen
    • major histocompatibility complex class I
    • major histocompatibility complex-peptide binding

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