Magnetoresistive sensor for real-time single nucleotide polymorphism genotyping

Giovanni Rizzi, Frederik Westergaard Østerberg, Martin Dufva, Mikkel Fougt Hansen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We demonstrate a magnetoresistive sensor platform that allows for the real-time detection of point mutations in DNA targets. Specifically, we detect point mutations at two sites in the human beta globin gene. For DNA detection, the present sensor technology has a detection limit of about 160pM and a dynamic range of about two orders of magnitude. The sensors are based on a new geometry for biological sensing that detects the difference between the amount of beads bound to a sensing pad and a local integrated negative reference pad. The magnetic beads are magnetised by the magnetic field arising from the sensor bias current such that no external magnetic fields are needed. The sensors are integrated in a microfluidic system with temperature control. The local negative reference integrated in the sensor geometry efficiently compensates for sensor offsets, external magnetic fields and a uniform background of magnetic beads, which enables real-time quantification of the specific binding of magnetic beads to the sensor surface under varying experimental conditions.
    Original languageEnglish
    JournalBiosensors and Bioelectronics
    Volume52
    Pages (from-to)445-451
    ISSN0956-5663
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Biosensor
    • Magnetoresistive sensor
    • Single nucleotide polymorphism
    • Microfluidics
    • DNA genotyping

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