TY - JOUR
T1 - Magnetoresistive sensor for real-time single nucleotide polymorphism genotyping
AU - Rizzi, Giovanni
AU - Østerberg, Frederik Westergaard
AU - Dufva, Martin
AU - Hansen, Mikkel Fougt
PY - 2014
Y1 - 2014
N2 - We demonstrate a magnetoresistive sensor platform that allows for the real-time detection of point mutations in DNA targets. Specifically, we detect point mutations at two sites in the human beta globin gene. For DNA detection, the present sensor technology has a detection limit of about 160pM and a dynamic range of about two orders of magnitude. The sensors are based on a new geometry for biological sensing that detects the difference between the amount of beads bound to a sensing pad and a local integrated negative reference pad. The magnetic beads are magnetised by the magnetic field arising from the sensor bias current such that no external magnetic fields are needed. The sensors are integrated in a microfluidic system with temperature control. The local negative reference integrated in the sensor geometry efficiently compensates for sensor offsets, external magnetic fields and a uniform background of magnetic beads, which enables real-time quantification of the specific binding of magnetic beads to the sensor surface under varying experimental conditions.
AB - We demonstrate a magnetoresistive sensor platform that allows for the real-time detection of point mutations in DNA targets. Specifically, we detect point mutations at two sites in the human beta globin gene. For DNA detection, the present sensor technology has a detection limit of about 160pM and a dynamic range of about two orders of magnitude. The sensors are based on a new geometry for biological sensing that detects the difference between the amount of beads bound to a sensing pad and a local integrated negative reference pad. The magnetic beads are magnetised by the magnetic field arising from the sensor bias current such that no external magnetic fields are needed. The sensors are integrated in a microfluidic system with temperature control. The local negative reference integrated in the sensor geometry efficiently compensates for sensor offsets, external magnetic fields and a uniform background of magnetic beads, which enables real-time quantification of the specific binding of magnetic beads to the sensor surface under varying experimental conditions.
KW - Biosensor
KW - Magnetoresistive sensor
KW - Single nucleotide polymorphism
KW - Microfluidics
KW - DNA genotyping
U2 - 10.1016/j.bios.2013.09.026
DO - 10.1016/j.bios.2013.09.026
M3 - Journal article
C2 - 24094523
SN - 0956-5663
VL - 52
SP - 445
EP - 451
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -