Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing

Cláudia Sofia Goncalves Gomes, Adedayo Fashina, Alfred Fernández-Castané, Timothy W Overton, Timothy John Hobley, Eirini Theodosiou*, Owen R. T. Thomas

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

BACKGROUND: The extraction of biopharmaceuticals from plasma and serum often employs overly complicated antiquated procedures that can inflict serious damage on especially prone protein targets and which afford low purification power and overall yields. This paper describes systematic development of a high‐gradient magnetic fishing process for recovery of immunoglobulins from unclarified antiserum.RESULTS: Non‐porous superparamagnetic particles were transformed into hydrophobic‐charge induction adsorbents and then used to recover immunoglobulins from rabbit antiserum feedstocks. Comprehensive characterisation tests conducted with variously diluted clarified antiserum on a magnetic rack revealed that immunoglobulin binding was rapid (equilibrium reached in <45 s), strong (Kd < 0.1 mg mL‐1), of high capacity (Qmax = 214 mg g‐1), and pH and ionic strength dependent. In a high‐gradient magnetic fishing process conducted with the same adsorbent, and a conventional ‘magnetic filter + recycle loop’ arrangement, >72% of the immunoglobulin present in an unclarified antiserum feed was recovered in 0.5 h in >3‐fold purified form.CONCLUSIONS: Fast magnetic particle based capture of antibodies from an unclarified high‐titre feed has been demonstrated. Efficient product recovery from ultra‐high titre bioprocess liquors by high‐gradient magnetic fishing requires that improved magnetic adsorbents displaying high selectivity, ultra‐high capacity and operational robustness are used with ‘state‐of‐the‐art’ rotor–stator magnetic separators. 
Original languageEnglish
JournalJournal of Chemical Technology and Biotechnology
Volume93
Issue number7
Pages (from-to)1901-1915
ISSN0268-2575
DOIs
Publication statusPublished - 2018

Keywords

  • 4-mercaptoethylpyridine
  • Complex unclarified bioprocess liquors
  • Direct capture
  • Polyclonal and monoclonal antibodies
  • Serum and plasma

Cite this

Gomes, Cláudia Sofia Goncalves ; Fashina, Adedayo ; Fernández-Castané, Alfred ; Overton, Timothy W ; Hobley, Timothy John ; Theodosiou, Eirini ; Thomas, Owen R. T. / Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing. In: Journal of Chemical Technology and Biotechnology. 2018 ; Vol. 93, No. 7. pp. 1901-1915.
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title = "Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing",
abstract = "BACKGROUND: The extraction of biopharmaceuticals from plasma and serum often employs overly complicated antiquated procedures that can inflict serious damage on especially prone protein targets and which afford low purification power and overall yields. This paper describes systematic development of a high‐gradient magnetic fishing process for recovery of immunoglobulins from unclarified antiserum.RESULTS: Non‐porous superparamagnetic particles were transformed into hydrophobic‐charge induction adsorbents and then used to recover immunoglobulins from rabbit antiserum feedstocks. Comprehensive characterisation tests conducted with variously diluted clarified antiserum on a magnetic rack revealed that immunoglobulin binding was rapid (equilibrium reached in <45 s), strong (Kd < 0.1 mg mL‐1), of high capacity (Qmax = 214 mg g‐1), and pH and ionic strength dependent. In a high‐gradient magnetic fishing process conducted with the same adsorbent, and a conventional ‘magnetic filter + recycle loop’ arrangement, >72{\%} of the immunoglobulin present in an unclarified antiserum feed was recovered in 0.5 h in >3‐fold purified form.CONCLUSIONS: Fast magnetic particle based capture of antibodies from an unclarified high‐titre feed has been demonstrated. Efficient product recovery from ultra‐high titre bioprocess liquors by high‐gradient magnetic fishing requires that improved magnetic adsorbents displaying high selectivity, ultra‐high capacity and operational robustness are used with ‘state‐of‐the‐art’ rotor–stator magnetic separators. ",
keywords = "4-mercaptoethylpyridine, Complex unclarified bioprocess liquors, Direct capture, Polyclonal and monoclonal antibodies, Serum and plasma",
author = "Gomes, {Cl{\'a}udia Sofia Goncalves} and Adedayo Fashina and Alfred Fern{\'a}ndez-Castan{\'e} and Overton, {Timothy W} and Hobley, {Timothy John} and Eirini Theodosiou and Thomas, {Owen R. T.}",
year = "2018",
doi = "10.1002/jctb.5599",
language = "English",
volume = "93",
pages = "1901--1915",
journal = "Journal of Chemical Technology and Biotechnology",
issn = "0268-2575",
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Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing. / Gomes, Cláudia Sofia Goncalves; Fashina, Adedayo; Fernández-Castané, Alfred; Overton, Timothy W; Hobley, Timothy John; Theodosiou, Eirini; Thomas, Owen R. T.

In: Journal of Chemical Technology and Biotechnology, Vol. 93, No. 7, 2018, p. 1901-1915.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Magnetic hydrophobic-charge induction adsorbents for the recovery of immunoglobulins from antiserum feedstocks by high-gradient magnetic fishing

AU - Gomes, Cláudia Sofia Goncalves

AU - Fashina, Adedayo

AU - Fernández-Castané, Alfred

AU - Overton, Timothy W

AU - Hobley, Timothy John

AU - Theodosiou, Eirini

AU - Thomas, Owen R. T.

PY - 2018

Y1 - 2018

N2 - BACKGROUND: The extraction of biopharmaceuticals from plasma and serum often employs overly complicated antiquated procedures that can inflict serious damage on especially prone protein targets and which afford low purification power and overall yields. This paper describes systematic development of a high‐gradient magnetic fishing process for recovery of immunoglobulins from unclarified antiserum.RESULTS: Non‐porous superparamagnetic particles were transformed into hydrophobic‐charge induction adsorbents and then used to recover immunoglobulins from rabbit antiserum feedstocks. Comprehensive characterisation tests conducted with variously diluted clarified antiserum on a magnetic rack revealed that immunoglobulin binding was rapid (equilibrium reached in <45 s), strong (Kd < 0.1 mg mL‐1), of high capacity (Qmax = 214 mg g‐1), and pH and ionic strength dependent. In a high‐gradient magnetic fishing process conducted with the same adsorbent, and a conventional ‘magnetic filter + recycle loop’ arrangement, >72% of the immunoglobulin present in an unclarified antiserum feed was recovered in 0.5 h in >3‐fold purified form.CONCLUSIONS: Fast magnetic particle based capture of antibodies from an unclarified high‐titre feed has been demonstrated. Efficient product recovery from ultra‐high titre bioprocess liquors by high‐gradient magnetic fishing requires that improved magnetic adsorbents displaying high selectivity, ultra‐high capacity and operational robustness are used with ‘state‐of‐the‐art’ rotor–stator magnetic separators. 

AB - BACKGROUND: The extraction of biopharmaceuticals from plasma and serum often employs overly complicated antiquated procedures that can inflict serious damage on especially prone protein targets and which afford low purification power and overall yields. This paper describes systematic development of a high‐gradient magnetic fishing process for recovery of immunoglobulins from unclarified antiserum.RESULTS: Non‐porous superparamagnetic particles were transformed into hydrophobic‐charge induction adsorbents and then used to recover immunoglobulins from rabbit antiserum feedstocks. Comprehensive characterisation tests conducted with variously diluted clarified antiserum on a magnetic rack revealed that immunoglobulin binding was rapid (equilibrium reached in <45 s), strong (Kd < 0.1 mg mL‐1), of high capacity (Qmax = 214 mg g‐1), and pH and ionic strength dependent. In a high‐gradient magnetic fishing process conducted with the same adsorbent, and a conventional ‘magnetic filter + recycle loop’ arrangement, >72% of the immunoglobulin present in an unclarified antiserum feed was recovered in 0.5 h in >3‐fold purified form.CONCLUSIONS: Fast magnetic particle based capture of antibodies from an unclarified high‐titre feed has been demonstrated. Efficient product recovery from ultra‐high titre bioprocess liquors by high‐gradient magnetic fishing requires that improved magnetic adsorbents displaying high selectivity, ultra‐high capacity and operational robustness are used with ‘state‐of‐the‐art’ rotor–stator magnetic separators. 

KW - 4-mercaptoethylpyridine

KW - Complex unclarified bioprocess liquors

KW - Direct capture

KW - Polyclonal and monoclonal antibodies

KW - Serum and plasma

U2 - 10.1002/jctb.5599

DO - 10.1002/jctb.5599

M3 - Journal article

VL - 93

SP - 1901

EP - 1915

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 7

ER -