Lymphatic fatty acids in canine with pharmaceutical formulations containing structured triacylglycerols

R. Holm, Trine Porsgaard, C.J.H. Porter, Carl-Erik Høy, G.A. Edwards, A. Mullertz, H.G. Kristensen, W.N. Charman

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The intramolecular structure of dietary triacylglycerols (TAG) influences absorption. In this study, two different pharmaceutical formulations were compared containing TAG differing in fatty acid profiles and intramolecular structures: LML and MLM, where M represented medium-chain fatty acids (MCFA; 8:0) and L represented long-chain fatty acids (LCFA). Lymph was collected from thoracic duct-cannulated canines for 12 h and the fatty acid composition was determined. The lymphatic transport of total fatty acids was significantly higher than the amount dosed; hence, the small exogenously dosed lipid recruited a large pool of endogenous fatty acids. The LML vehicle led to a significantly higher total fatty acid transport than the MLM vehicle. The amount of 8:0 recovered in lymph was almost similar and low for both groups. The amount of LCFA recovered from the animals dosed with the LML vehicle was generally higher than from the animals dosed with the MLM vehicle; however, statistically significant differences were only found for 18:0 and 18:3n-3. In conclusion, these results indicated that the fatty acid profile and intramolecular structure of administered TAG influenced the absorption of fatty acids in canines, also when the TAG was incorporated into a pharmaceutical formulation in low amounts.
    Original languageEnglish
    JournalEuropean Journal of Lipid Science and Technology
    Volume108
    Issue number9
    Pages (from-to)714-722
    ISSN1438-7697
    Publication statusPublished - 2006

    Keywords

    • canine
    • structured triacylglycerols
    • lymphatic transport
    • pharmaceutical formulation

    Cite this

    Holm, R., Porsgaard, T., Porter, C. J. H., Høy, C-E., Edwards, G. A., Mullertz, A., Kristensen, H. G., & Charman, W. N. (2006). Lymphatic fatty acids in canine with pharmaceutical formulations containing structured triacylglycerols. European Journal of Lipid Science and Technology, 108(9), 714-722.