Luminescence from lanthanide(III) ions bound to the Glycocalyx of Chinese Hamster Ovary cells

Riikka Arppe-Tabbara, Miguel R Carro-Temboury, Casper Hempel*, Tom Vosch, Thomas Just Sørensen

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Lanthanide(III) ions bind to the glycocalyx of Chinese Hamster Ovary (CHO) cells and give rise to a unique luminescent fingerprint. Following direct excitation of terbium(III) or europium(III) ions in the visible part of the spectrum, we are able to collect emission spectra pixel-by-pixel in images of CHO cells. Following data analysis that removes the background signal, the fine structure of the europium(III) luminescence indicate that the lanthanide(III) ions are bound to a single structure of the CHO cell glycocalyx. This was deduced from the fact that the structure-sensitive emission spectrum of europium is unchanged throughout the investigated
    samples.
    Original languageEnglish
    JournalChemistry: A European Journal
    Volume24
    Issue number46
    Pages (from-to)11885-11889
    ISSN0947-6539
    DOIs
    Publication statusPublished - 2018

    Cite this

    Arppe-Tabbara, Riikka ; Carro-Temboury, Miguel R ; Hempel, Casper ; Vosch, Tom ; Sørensen, Thomas Just. / Luminescence from lanthanide(III) ions bound to the Glycocalyx of Chinese Hamster Ovary cells. In: Chemistry: A European Journal. 2018 ; Vol. 24, No. 46. pp. 11885-11889.
    @article{9e3526b604204df1a643be63dd40ba9a,
    title = "Luminescence from lanthanide(III) ions bound to the Glycocalyx of Chinese Hamster Ovary cells",
    abstract = "Lanthanide(III) ions bind to the glycocalyx of Chinese Hamster Ovary (CHO) cells and give rise to a unique luminescent fingerprint. Following direct excitation of terbium(III) or europium(III) ions in the visible part of the spectrum, we are able to collect emission spectra pixel-by-pixel in images of CHO cells. Following data analysis that removes the background signal, the fine structure of the europium(III) luminescence indicate that the lanthanide(III) ions are bound to a single structure of the CHO cell glycocalyx. This was deduced from the fact that the structure-sensitive emission spectrum of europium is unchanged throughout the investigatedsamples.",
    author = "Riikka Arppe-Tabbara and Carro-Temboury, {Miguel R} and Casper Hempel and Tom Vosch and S{\o}rensen, {Thomas Just}",
    year = "2018",
    doi = "10.1002/chem.201802799",
    language = "English",
    volume = "24",
    pages = "11885--11889",
    journal = "Chemistry: A European Journal",
    issn = "0947-6539",
    publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
    number = "46",

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    Luminescence from lanthanide(III) ions bound to the Glycocalyx of Chinese Hamster Ovary cells. / Arppe-Tabbara, Riikka; Carro-Temboury, Miguel R; Hempel, Casper; Vosch, Tom; Sørensen, Thomas Just.

    In: Chemistry: A European Journal, Vol. 24, No. 46, 2018, p. 11885-11889.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Luminescence from lanthanide(III) ions bound to the Glycocalyx of Chinese Hamster Ovary cells

    AU - Arppe-Tabbara, Riikka

    AU - Carro-Temboury, Miguel R

    AU - Hempel, Casper

    AU - Vosch, Tom

    AU - Sørensen, Thomas Just

    PY - 2018

    Y1 - 2018

    N2 - Lanthanide(III) ions bind to the glycocalyx of Chinese Hamster Ovary (CHO) cells and give rise to a unique luminescent fingerprint. Following direct excitation of terbium(III) or europium(III) ions in the visible part of the spectrum, we are able to collect emission spectra pixel-by-pixel in images of CHO cells. Following data analysis that removes the background signal, the fine structure of the europium(III) luminescence indicate that the lanthanide(III) ions are bound to a single structure of the CHO cell glycocalyx. This was deduced from the fact that the structure-sensitive emission spectrum of europium is unchanged throughout the investigatedsamples.

    AB - Lanthanide(III) ions bind to the glycocalyx of Chinese Hamster Ovary (CHO) cells and give rise to a unique luminescent fingerprint. Following direct excitation of terbium(III) or europium(III) ions in the visible part of the spectrum, we are able to collect emission spectra pixel-by-pixel in images of CHO cells. Following data analysis that removes the background signal, the fine structure of the europium(III) luminescence indicate that the lanthanide(III) ions are bound to a single structure of the CHO cell glycocalyx. This was deduced from the fact that the structure-sensitive emission spectrum of europium is unchanged throughout the investigatedsamples.

    U2 - 10.1002/chem.201802799

    DO - 10.1002/chem.201802799

    M3 - Journal article

    VL - 24

    SP - 11885

    EP - 11889

    JO - Chemistry: A European Journal

    JF - Chemistry: A European Journal

    SN - 0947-6539

    IS - 46

    ER -