Low-voltage stimulated denitrification performance of high-salinity wastewater using halotolerant microorganisms

Long Chen, Hai Xiang, Li-Ting Zhou, Yan-Qing Zhang, Yang-Cheng Ding, Di Wu, Nan-Wen Zhu, Yi-Feng Zhang, Hua-Jun Feng*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Nitrate is a common contaminant in high-salinity wastewater, which has adverse effects on both the environment and human health. However, conventional biological treatment exhibits poor denitrification performance due to the high-salinity shock. In this study, an innovative approach using an electrostimulating microbial reactor (EMR) was explored to address this challenge. With a low-voltage input of 1.2 V, the EMR reached nitrate removal kinetic parameter (kNO3-N) of 0.0166–0.0808 h−1 under high-salinities (1.5 %-6.5 %), which was higher than that of the microbial reactor (MR) (0.0125–0.0478 h−1). The mechanisms analysis revealed that low-voltage significantly enhanced microbial salt-in strategy and promoted the secretion of extracellular polymeric substances. Halotolerant denitrification microorganisms (Pseudomonas and Nitratireductor) were also enriched in EMR. Moreover, the EMR achieved a NO3-N removal efficiency of 73.64 % in treating high-salinity wastewater (salinity 4.69 %) over 18-cycles, whereas the MR only reached 54.67 %. In summary, this study offers an innovative solution for denitrification of high-salinity wastewater
Original languageEnglish
Article number130688
JournalBioresource Technology
Number of pages9
Publication statusPublished - 2024


  • High-salinity wastewater
  • Low-voltage stimulation
  • Salt-tolerance
  • Denitrification


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