Low translocation of Bacillus thuringiensis israelensis to inner organs in mice after pulmonary exposure to commercial biopesticide

Kenneth Klingenberg Barfod, Lasse Ørum-Smidt, Karen A. Krogfelt, Søren Thor Larsen, Lars Andrup

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Translocation of viable cells from a Bacillus thuringiensis israelensis-based biopesticide to inner organs in a mouse model was studied. Mice were exposed to the originally formulated product through the lungs and gastrointestinal tract by intratracheal instillation. Colony forming units (CFU) were grown from lungs, caecum, spleen and liver on Bacillus cereus-specific agar (BCSA) after 24 h and finally determined to be biopesticide strain B. t. israelensis by large plasmid profile. No CFU were found in spleen or liver of the control mice or in any aerosol background or material. We have shown that viable cells from the commercial product can translocate to spleen and liver of immunocompetent mice in a dose-dependent manner. Furthermore, we discuss the methods of exposure and how bacterial translocation should be taken into consideration when evaluating the safety of novel or reintroduced biopesticides in the future.
Original languageEnglish
JournalBiocontrol Science and Technology
Volume20
Issue number9
Pages (from-to)1001 - 1011
ISSN0958-3157
DOIs
Publication statusPublished - 2010

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