Longitudinal study of interferon-gamma, serum antibody and milk antibody responses in cattle infected with Mycobacterium avium subsp paratuberculosis

A. Huda, Gregers Jungersen, Peter Lind

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    During a 2-year study period, 252 animals from dairy herds infected with Mycobacterium avium subsp. paratuberculosis, and 119 animals from non-infected herds were subjected to repeated blood and faecal sampling. Animals were retrospectively grouped by infection status as infected, exposed (culture negative animals from infected herds), or non-infected animals, and by age, 12-23 months (1+ year), 24-35 months (2+ years), or 36 months and older (3+ years). Samples were collected for culture of faeces, assessment of interferon-gamma (IFN-gamma) secreted by M. paratuberculosis antigen stimulated whole-blood lymphocytes (IFN-gamma test), and measurement of antibody responses against M. paratuberculosis in serum and milk by an in-house absorbed ELISA. The IFN-gamma test diagnosed higher proportions of infected and exposed animals than the antibody ELISAs. The highest sensitivity of IFN-gamma test was in infected cattle of 2+ years of age. Receiver-operating characteristic (ROC) analyses supported the assumption that the IFN-gamma test had a better performance than antibody tests of animals of 1+ and 2+ years of age. However, for animals of 3+ years all tests performed equally well. Application of single sampling compared with repeated samplings showed better performance of the IFN-gamma test by repeated samplings, and the milk antibody ELISA in animals of 3+ years of age performed significantly better with repeated sampling compared with single sampling. In conclusion, the IFN-gamma test may be applied for screening of cattle of I and 2 years of age for exposure to M. paratuberculosis and the antibody ELISAs from 3 years of age.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume104
    Issue number1-2
    Pages (from-to)43-53
    ISSN0378-1135
    DOIs
    Publication statusPublished - 2004

    Keywords

    • longitudinal study
    • Mycobacterium avium subsp paratuberculosis
    • bacteria
    • interferon-gamma
    • paratuberculosis
    • cattle
    • ROC analysis

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