Abstract
Microbial communities derived from soils subject to different agronomic treatments were challenged with three broad host range plasmids, RP4, pIPO2tet and pRO101, via solid surface filter matings to assess their permissiveness. Approximately 1 in 10 000 soil bacterial cells could receive and maintain the plasmids. The community permissiveness increased up to 100% in communities derived from manured soil. While the plasmid transfer frequency was significantly influenced by both the type of plasmid and the agronomic treatment, the diversity of the transconjugal pools was purely plasmid dependent and was dominated by β- and γ-Proteobacteria.
| Original language | English |
|---|---|
| Journal | Environmental Microbiology Reports |
| Volume | 6 |
| Issue number | 2 |
| Pages (from-to) | 125-130 |
| Number of pages | 6 |
| ISSN | 1758-2229 |
| DOIs | |
| Publication status | Published - 2014 |
Keywords
- SOIL microbiology
- GenBank sequence data
- manure exposure
- soil bacterial community potential
- Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] beta-Proteobacteria higher_taxa gamma-Proteobacteria higher_taxa
- pIPO2tet
- plasmid
- pRO101
- RP4
- 03502, Genetics - General
- 07502, Ecology: environmental biology - General and methods
- 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
- 30000, Bacteriology, general and systematic
- 31000, Physiology and biochemistry of bacteria
- 31500, Genetics of bacteria and viruses
- Biochemistry and Molecular Biophysics
- agronomic treatment applied and field techniques
- Biodiversity
- Molecular Genetics
- ENVIRONMENTAL
- MICROBIOLOGY
- BROAD-HOST-RANGE
- HORIZONTAL GENE-TRANSFER
- RESISTANT BACTERIA
- WHEAT RHIZOSPHERE
- PERMISSIVENESS
- FERTILIZATION
- PSEUDOMONAS
- RESIDUES
- ELEMENTS