Long-term brain slice culturing in a microfluidic platform

Indumathi Vedarethinam; N. Avaliani; J. Tønnesen; J. Hansen; David Sabourin; Maria Dimaki; M. Kokaia; Martin Dufva; Winnie Edith Svendsen; Jenny Emnéus; Arto Heiskanen

Long-term brain slice culturing in a microfluidic platform

Indumathi Vedarethinam, N. Avaliani, J. Tønnesen, J. Hansen, David Sabourin, Maria Dimaki, M. Kokaia, Martin Dufva, Winnie Edith Svendsen, Jenny Emnéus, Arto Heiskanen

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Abstract

In this work, we present the development of a transparent poly(methyl methacrylate) (PMMA) based microfluidic culture system for handling long-term brain slice cultures independent of an incubator. The different stages of system development have been validated by culturing GFP producing brain slices from 8-day old (P8) mouse pups. Fluorescence microscopic monitoring of GFP was utilized as an indicator of tissue viability. The final format of the developed system, featuring ”plug-and-play” technolgy with a reusable fluidic connection board and easily changeable microfluidic chips, facilitated brain slice culturing for 16 days.

Original language	English
Title of host publication	Proceedings of International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publication date	2011
Pages	1560-1563
Publication status	Published - 2011
Event	37th International Conference on Micro and Nano Engineering - Berlin, Germany Duration: 19 Sept 2011 → 23 Sept 2011 Conference number: 37 http://www.mne-conf.org/GENERAL/index.php

Conference

Conference	37th International Conference on Micro and Nano Engineering
Number	37
Country/Territory	Germany
City	Berlin
Period	19/09/2011 → 23/09/2011
Internet address	http://www.mne-conf.org/GENERAL/index.php

Keywords

Microfluidic Tissue Culture
Brain Slice
Long-Term Culture
Stem Cell Therapy

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@inproceedings{242085566df04ea09ef7506c89473a9e,

title = "Long-term brain slice culturing in a microfluidic platform",

abstract = "In this work, we present the development of a transparent poly(methyl methacrylate) (PMMA) based microfluidic culture system for handling long-term brain slice cultures independent of an incubator. The different stages of system development have been validated by culturing GFP producing brain slices from 8-day old (P8) mouse pups. Fluorescence microscopic monitoring of GFP was utilized as an indicator of tissue viability. The final format of the developed system, featuring ”plug-and-play” technolgy with a reusable fluidic connection board and easily changeable microfluidic chips, facilitated brain slice culturing for 16 days.",

keywords = "Microfluidic Tissue Culture, Brain Slice, Long-Term Culture, Stem Cell Therapy",

author = "Indumathi Vedarethinam and N. Avaliani and J. T{\o}nnesen and J. Hansen and David Sabourin and Maria Dimaki and M. Kokaia and Martin Dufva and Svendsen, {Winnie Edith} and Jenny Emn{\'e}us and Arto Heiskanen",

year = "2011",

language = "English",

pages = "1560--1563",

booktitle = "Proceedings of International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "37th International Conference on Micro and Nano Engineering, MNE 2011 ; Conference date: 19-09-2011 Through 23-09-2011",

url = "http://www.mne-conf.org/GENERAL/index.php",

}

Vedarethinam, I, Avaliani, N, Tønnesen, J, Hansen, J, Sabourin, D, Dimaki, M, Kokaia, M, Dufva, M , Svendsen, WE , Emnéus, J & Heiskanen, A 2011, Long-term brain slice culturing in a microfluidic platform. in Proceedings of International Conference on Miniaturized Systems for Chemistry and Life Sciences. pp. 1560-1563, 37th International Conference on Micro and Nano Engineering, Berlin, Germany, 19/09/2011.

TY - GEN

T1 - Long-term brain slice culturing in a microfluidic platform

AU - Vedarethinam, Indumathi

AU - Avaliani, N.

AU - Tønnesen, J.

AU - Hansen, J.

AU - Sabourin, David

AU - Dimaki, Maria

AU - Kokaia, M.

AU - Dufva, Martin

AU - Svendsen, Winnie Edith

AU - Emnéus, Jenny

AU - Heiskanen, Arto

N1 - Conference code: 37

PY - 2011

Y1 - 2011

N2 - In this work, we present the development of a transparent poly(methyl methacrylate) (PMMA) based microfluidic culture system for handling long-term brain slice cultures independent of an incubator. The different stages of system development have been validated by culturing GFP producing brain slices from 8-day old (P8) mouse pups. Fluorescence microscopic monitoring of GFP was utilized as an indicator of tissue viability. The final format of the developed system, featuring ”plug-and-play” technolgy with a reusable fluidic connection board and easily changeable microfluidic chips, facilitated brain slice culturing for 16 days.

AB - In this work, we present the development of a transparent poly(methyl methacrylate) (PMMA) based microfluidic culture system for handling long-term brain slice cultures independent of an incubator. The different stages of system development have been validated by culturing GFP producing brain slices from 8-day old (P8) mouse pups. Fluorescence microscopic monitoring of GFP was utilized as an indicator of tissue viability. The final format of the developed system, featuring ”plug-and-play” technolgy with a reusable fluidic connection board and easily changeable microfluidic chips, facilitated brain slice culturing for 16 days.

KW - Microfluidic Tissue Culture

KW - Brain Slice

KW - Long-Term Culture

KW - Stem Cell Therapy

M3 - Article in proceedings

SP - 1560

EP - 1563

BT - Proceedings of International Conference on Miniaturized Systems for Chemistry and Life Sciences

T2 - 37th International Conference on Micro and Nano Engineering

Y2 - 19 September 2011 through 23 September 2011

ER -

Long-term brain slice culturing in a microfluidic platform

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Keywords

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