Long-term brain slice culturing in a microfluidic platform

Indumathi Vedarethinam, N. Avaliani, J. Tønnesen, J. Hansen, David Sabourin, Maria Dimaki, M. Kokaia, Martin Dufva, Winnie Edith Svendsen, Jenny Emnéus, Arto Heiskanen

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    Abstract

    In this work, we present the development of a transparent poly(methyl methacrylate) (PMMA) based microfluidic culture system for handling long-term brain slice cultures independent of an incubator. The different stages of system development have been validated by culturing GFP producing brain slices from 8-day old (P8) mouse pups. Fluorescence microscopic monitoring of GFP was utilized as an indicator of tissue viability. The final format of the developed system, featuring ”plug-and-play” technolgy with a reusable fluidic connection board and easily changeable microfluidic chips, facilitated brain slice culturing for 16 days.
    Original languageEnglish
    Title of host publicationProceedings of International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Publication date2011
    Pages1560-1563
    Publication statusPublished - 2011
    Event37th International Conference on Micro and Nano Engineering - Berlin, Germany
    Duration: 19 Sep 201123 Sep 2011
    Conference number: 37
    http://www.mne-conf.org/GENERAL/index.php

    Conference

    Conference37th International Conference on Micro and Nano Engineering
    Number37
    CountryGermany
    CityBerlin
    Period19/09/201123/09/2011
    Internet address

    Keywords

    • Microfluidic Tissue Culture
    • Brain Slice
    • Long-Term Culture
    • Stem Cell Therapy

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