Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

Louise K. Johansen, Tine M. Iburg, Ole L. Nielsen, Páll S. Leifsson, Kirstin Dahl-Petersen, Janne Koch, Dorte Frees, Bent Aalbæk, Peter M.H. Heegaard, Henrik E. Jensen

    Research output: Contribution to journalJournal articleResearchpeer-review


    It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6h, 12h, 24h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.
    Original languageEnglish
    JournalProstaglandins & Other Lipid Mediators
    Issue number3-4
    Pages (from-to)103-108
    Publication statusPublished - 2012


    • Osteomyelitis
    • Bone morphology
    • Cyclooxygenase 2
    • Alkaline phosphatase
    • Haptoglobin
    • Porcine models


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