LNA for Optimization of Fluorescent Oligonucleotide Probes: Improved Spectral Properties and Target Binding

Kira Astakhova, Alexey V. Ustinov, Vladimir A. Korshun, Jesper Wengel

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2'-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.
Original languageEnglish
JournalBioconjugate Chemistry
Volume22
Issue number4
Pages (from-to)533-539
Number of pages7
ISSN1043-1802
DOIs
Publication statusPublished - 2011
Externally publishedYes

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