Liquid carry-over in an injection moulded all-polymer chip system for immiscible phase magnetic bead-based solid-phase extraction

Kasper Kistrup, Karen Skotte Sørensen, Anders Wolff, Mikkel Fougt Hansen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We present an all-polymer, single-use microfluidic chip system produced by injection moulding and bonded by ultrasonic welding. Both techniques are compatible with low-cost industrial mass-production. The chip is produced for magnetic bead-based solid-phase extraction facilitated by immiscible phase filtration and features passive liquid filling and magnetic bead manipulation using an external magnet. In this work, we determine the system compatibility with various surfactants. Moreover, we quantify the volume of liquid co-transported with magnetic bead clusters from Milli-Q water or a lysis-binding buffer for nucleic acid extraction (0.1 (v/v)% Triton X-100 in 5 M guanidine hydrochloride). A linear relationship was found between the liquid carry-over and mass of magnetic beads used. Interestingly, similar average carry-overs of 1.74(8) nL/µg and 1.72(14) nL/µg were found for Milli-Q water and lysis-binding buffer, respectively.
    Original languageEnglish
    JournalJournal of Magnetism and Magnetic Materials
    Volume380
    Pages (from-to)191-196
    Number of pages6
    ISSN0304-8853
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Polymer microfluidics
    • Magnetic beads
    • Injection moulding
    • Ultrasonic welding
    • Immiscible phase filtration
    • Capillary stop
    • Mobile solid-phase extraction

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