Lipophilic antioxidants and polyunsaturated fatty acids in lipoprotein classes: distribution and interaction

V.H. Sunesen, Christine Weber, Gunhild Kofoed Hølmer

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    Abstract

    Objective: To study the lipoprotein distribution of supplemented coenzyme Q(10) (CoQ(10)), vitamin E, and polyunsaturated fatty acids (PUFA). Design: Balanced three- period crossover study. Setting: University research unit. Subjects: Eighteen apparently healthy free-living non-smoking volunteers (nine women, nine men), mean age 26 +/- 3 y, recruited among the university students; no dropouts. Interventions: Three supplementation periods of 10 days: 100 m/day CoQ(10), 350 mg/day D-alpha -tocopherol, and 2g/day concentrated fish oil. Fasting venous blood samples were collected twice before the first period and then after each period. Plasma and isolated lipoproteins were analysed for cholesterol, triacylglycerol, alpha- and gamma -tocopherol, CoQ(10), and fatty acid composition. Results: Significant (P <0.05) increase in CoQ(10) and -tocopherol occurred in all lipoprotein classes after supplementation. CoQ(10) was primarily incorporated into low-density lipoprotein (LDL). alpha -tocopherol and fish oil n-3 PUFAs had similar patterns. They were equally distributed between LDL and high-density lipoprotein (HDL), with a smaller part in VLDL. The total sum of PUFA was unchanged following all supplementations, but fish oil increased the amount of n-3 fatty acids at the expense of n-6 fatty acids. Conclusion: Lipoprotein distribution of CoQ(10) is markedly different from that of alpha -tocopherol, suggesting that they may be metabolised by distinct routes. alpha -Tocopherol is distributed similarly to n-3 fatty acids, thus providing protection on location for the oxidatively labile PUFAs.
    Original languageEnglish
    JournalEuropean Journal of Clinical Nutrition
    Volume55
    Issue number2
    Pages (from-to)115-123
    ISSN0954-3007
    Publication statusPublished - 2001

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