Lipase-catalysed synthesis of glucose fatty acid esters in tert-butanol

Peter Degn, Lars H. Pedersen, Jens Øllgaard Duus, Wolfgang Zimmermann

Research output: Contribution to journalJournal articleResearchpeer-review


Synthesis of 6-O-acylate-α-D-glycopyranose from underivatised substrates in anhydrous tert-butanol was achieved using immobilised lipases from Candida antarctica and Mucor miehei. Except for acetic acid, the initial reaction rates with the C. antarctica lipase were independent of acyl donor chain lengths and in a range of 3.9 ± 0.4 mol glucose converted min-1 g enzyme preparation. The catalytic activity of the M. miehei lipase increased with increasing acyl donor chain length with a maximum for stearic acid of 0.45 μmol min-1 g. Using maltose as substrate, the catalytic activity decreased by a factor of 48 and 20 with the lipase from C. antarctica and M. miehei, respectively, while with maltotriose no reaction was observed.
Original languageEnglish
JournalBiotechnology Letters
Pages (from-to)275–280
Publication statusPublished - 1999
Externally publishedYes


  • Fatty acid ester
  • Glucose
  • Lipase


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