Linking secondary metabolites to biosynthesis genes in the fungal endophyte Cyanodermella asteris: The anti-cancer bisanthraquinone skyrin

Linda Jahn, Thomas Schafhauser, Daniel Wibberg, Christian Rückert, Anika Winkler, Andreas Kulik, Tilmann Weber, Liane Flor, Karl-Heinz van Pée, Jörn Kalinowski, Jutta Ludwig-Müllera, Wolfgang Wohlleben

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Fungal aromatic polyketides display a very diverse and widespread group of natural products. Due to their excellent light absorption properties and widely studied biological activities, they offer numerous application for food, textile and pharmaceutical industry. The biosynthetic pathways of fungal aromatic polyketides usually involve a set of successive enzymes, in which a non-reductive polyketide synthases iteratively catalyzes the essential assembly of simple building blocks into (often polycyclic) aromatic compounds. However, only a limited number of such pathways have been described so far and further elucidation of the individual biosynthetic steps is needed to fully exploit the biotechnological and medicinal potential of these compounds. Here, we identified the bisanthraquinone skyrin as the main pigment of the fungus Cyanodermella asteris, an endophyte that has recently been isolated from the traditional Chinese medicinal plant Aster tataricus. The genome of C. asteris was sequenced, assembled and annotated, which enables first insights into a genome from a non-lichenized member of the class Lecanoromycetes. Genetic and in silico analysis led to the identification of a gene cluster of five genes suggested to encode the enzymatic pathway for skyrin. Our study is a starting point for rational pathway engineering in order to drive the production towards higher yields or more active derivatives. Moreover, our investigations revealed a large potential of secondary metabolite production in C. asteris as well as in all Lecanoromycetes genomes of which genomes were available. These findings convincingly emphasize that Lecanoromycetes are prolific producers of secondary metabolites.
Original languageEnglish
JournalJournal of Biotechnology
Volume257
Pages (from-to)233-239
ISSN0168-1656
DOIs
Publication statusPublished - 2017

Keywords

  • Anthraquinone
  • Lecanoromycetes
  • Biosynthetic gene cluster
  • Emodin
  • Endophyte
  • Skyrin

Cite this

Jahn, L., Schafhauser, T., Wibberg, D., Rückert, C., Winkler, A., Kulik, A., Weber, T., Flor, L., van Pée, K-H., Kalinowski, J., Ludwig-Müllera, J., & Wohlleben, W. (2017). Linking secondary metabolites to biosynthesis genes in the fungal endophyte Cyanodermella asteris: The anti-cancer bisanthraquinone skyrin. Journal of Biotechnology, 257, 233-239. https://doi.org/10.1016/j.jbiotec.2017.06.410