Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples

Research output: Contribution to journalJournal article – Annual report year: 2014Researchpeer-review

  • Author: van der Poel, W. H. M.

    Central Veterinary Institute, Netherlands

  • Author: Cay, B.

    Veterinary and Agrochemical Research Centre, Belgium

  • Author: Zientara, S

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Steinbach, F.

    Animal Health and Veterinary Laboratories Agency, United Kingdom

  • Author: Valarcher, Jean-François

    National Veterinary Institute, Sweden

  • Author: Bøtner, Anette

    Section for Virology, National Veterinary Institute, Technical University of Denmark, Lindholm, 4771, Kalvehave, Denmark

  • Author: Mars, Maria Henriette

    Animal Health Service, Netherlands

  • Author: Hakze-van der Honing, R.

    Central Veterinary Institute, Netherlands

  • Author: Schirrmeier, H.

    Friedrich-Loeffler-Institute, Germany

  • Author: Beer, M.

    Friedrich-Loeffler-Institute, Germany

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Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.
Original languageEnglish
Article number380
JournalVeterinary Record
Issue number15
Publication statusPublished - 2014
CitationsWeb of Science® Times Cited: No match on DOI

ID: 91573322