Limited dissemination of the wastewater treatment plant core resistome

Christian Munck; Mads Albertsen; Amar Telke; Mostafa M Hashim Ellabaan; Per Halkjær Nielsen; Morten Otto Alexander Sommer

doi:10.1038/ncomms9452

Limited dissemination of the wastewater treatment plant core resistome

Christian Munck, Mads Albertsen, Amar Telke, Mostafa M Hashim Ellabaan, Per Halkjær Nielsen, Morten Otto Alexander Sommer

Novo Nordisk Foundation Center for Biosustainability

Aalborg University

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Abstract

Horizontal gene transfer is a major contributor to the evolution of bacterial genomes and can facilitate the dissemination of antibiotic resistance genes between environmental reservoirs and potential pathogens. Wastewater treatment plants (WWTPs) are believed to play a central role in the dissemination of antibiotic resistance genes. However, the contribution of the dominant members of the WWTP resistome to resistance in human pathogens remains poorly understood. Here we use a combination of metagenomic functional selections and comprehensive metagenomic sequencing to uncover the dominant genes of the WWTP resistome. We find that this core resistome is unique to the WWTP environment, with <10% of the resistance genes found outside the WWTP environment. Our data highlight that, despite an abundance of functional resistance genes within WWTPs, only few genes are found in other environments, suggesting that the overall dissemination of the WWTP resistome is comparable to that of the soil resistome.

Original language	English
Article number	8452
Journal	Nature Communications
Volume	6
Number of pages	10
ISSN	2041-1723
DOIs	https://doi.org/10.1038/ncomms9452
Publication status	Published - 2015

Bibliographical note

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Keywords

Biological sciences
Ecology
Microbiology

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10.1038/ncomms9452

Limited dissemination of the wastewater treatmentFinal published version, 470 KB

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Elucidating antibiotic resistomes from soil and wastewater treatment plants
Sommer, M. O. A. (Invited speaker)
1 Dec 2015
Activity: Talks and presentations › Guest lectures, external teaching and course activities at other universities

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abstract = "Horizontal gene transfer is a major contributor to the evolution of bacterial genomes and can facilitate the dissemination of antibiotic resistance genes between environmental reservoirs and potential pathogens. Wastewater treatment plants (WWTPs) are believed to play a central role in the dissemination of antibiotic resistance genes. However, the contribution of the dominant members of the WWTP resistome to resistance in human pathogens remains poorly understood. Here we use a combination of metagenomic functional selections and comprehensive metagenomic sequencing to uncover the dominant genes of the WWTP resistome. We find that this core resistome is unique to the WWTP environment, with <10% of the resistance genes found outside the WWTP environment. Our data highlight that, despite an abundance of functional resistance genes within WWTPs, only few genes are found in other environments, suggesting that the overall dissemination of the WWTP resistome is comparable to that of the soil resistome.",

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AU - Nielsen, Per Halkjær

AU - Sommer, Morten Otto Alexander

N1 - This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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AB - Horizontal gene transfer is a major contributor to the evolution of bacterial genomes and can facilitate the dissemination of antibiotic resistance genes between environmental reservoirs and potential pathogens. Wastewater treatment plants (WWTPs) are believed to play a central role in the dissemination of antibiotic resistance genes. However, the contribution of the dominant members of the WWTP resistome to resistance in human pathogens remains poorly understood. Here we use a combination of metagenomic functional selections and comprehensive metagenomic sequencing to uncover the dominant genes of the WWTP resistome. We find that this core resistome is unique to the WWTP environment, with <10% of the resistance genes found outside the WWTP environment. Our data highlight that, despite an abundance of functional resistance genes within WWTPs, only few genes are found in other environments, suggesting that the overall dissemination of the WWTP resistome is comparable to that of the soil resistome.

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Limited dissemination of the wastewater treatment plant core resistome

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Elucidating antibiotic resistomes from soil and wastewater treatment plants

Cite this