Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers

Farizah Saharil; Annika Ahlford; Malte Kuhnemund; Maciej Skolimowski; Alvaro Conde; Martin Dufva; Mats Nilsson; Monica Brivio; Wouter van der Wijngaart; Tommy Haraldsson

doi:10.1109/Transducers.2013.6626776

Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers

Farizah Saharil
, Annika Ahlford
, Malte Kuhnemund
, Maciej Skolimowski
, Alvaro Conde
, Martin Dufva
, Mats Nilsson
, Monica Brivio
, Wouter van der Wijngaart
, Tommy Haraldsson

KTH Royal Institute of Technology
Stockholm University
Universidad Nacional de Tucuman

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.

Original language	English
Title of host publication	Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	IEEE
Publication date	2013
ISBN (Print)	9781467359832
DOIs	https://doi.org/10.1109/Transducers.2013.6626776
Publication status	Published - 2013
Event	17th International Conference on Solid-State Sensors, Actuators and Microsystems - Centro de Convenciones Internacional de Barcelona (CCIB) , Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013 Conference number: 17 http://transducers-eurosensors2013.org/

Conference

Conference	17th International Conference on Solid-State Sensors, Actuators and Microsystems
Number	17
Location	Centro de Convenciones Internacional de Barcelona (CCIB)
Country/Territory	Spain
City	Barcelona
Period	16/06/2013 → 20/06/2013
Internet address	http://transducers-eurosensors2013.org/

Keywords

DNA
lab-on-a-chip
microfluidics
molecular biophysics
polymers
stoichiometry
Computing and Processing
Engineering Profession
General Topics for Engineers
Geoscience
Nuclear Engineering

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10.1109/Transducers.2013.6626776

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Saharil, F., Ahlford, A., Kuhnemund, M., Skolimowski, M., Conde, A., Dufva, M., Nilsson, M., Brivio, M., van der Wijngaart, W., & Haraldsson, T. (2013). Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. In Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems IEEE. https://doi.org/10.1109/Transducers.2013.6626776

@inproceedings{543b0b782a3f47529ba10428917813b0,

title = "Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers",

abstract = "For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.",

keywords = "DNA, lab-on-a-chip, microfluidics, molecular biophysics, polymers, stoichiometry, Computing and Processing, Engineering Profession, General Topics for Engineers, Geoscience, Nuclear Engineering",

author = "Farizah Saharil and Annika Ahlford and Malte Kuhnemund and Maciej Skolimowski and Alvaro Conde and Martin Dufva and Mats Nilsson and Monica Brivio and \{van der Wijngaart\}, Wouter and Tommy Haraldsson",

year = "2013",

doi = "10.1109/Transducers.2013.6626776",

language = "English",

isbn = "9781467359832",

booktitle = "Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems",

publisher = "IEEE",

address = "United States",

note = "17th International Conference on Solid-State Sensors, Actuators and Microsystems ; Conference date: 16-06-2013 Through 20-06-2013",

url = "http://transducers-eurosensors2013.org/",

}

Saharil, F, Ahlford, A, Kuhnemund, M, Skolimowski, M, Conde, A, Dufva, M, Nilsson, M, Brivio, M, van der Wijngaart, W & Haraldsson, T 2013, Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. in Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems. IEEE, 17th International Conference on Solid-State Sensors, Actuators and Microsystems , Barcelona, Spain, 16/06/2013. https://doi.org/10.1109/Transducers.2013.6626776

Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. / Saharil, Farizah; Ahlford, Annika; Kuhnemund, Malte et al.
Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems. IEEE, 2013.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers

AU - Saharil, Farizah

AU - Ahlford, Annika

AU - Kuhnemund, Malte

AU - Skolimowski, Maciej

AU - Conde, Alvaro

AU - Dufva, Martin

AU - Nilsson, Mats

AU - Brivio, Monica

AU - van der Wijngaart, Wouter

AU - Haraldsson, Tommy

N1 - Conference code: 17

PY - 2013

Y1 - 2013

N2 - For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.

AB - For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.

KW - DNA

KW - lab-on-a-chip

KW - microfluidics

KW - molecular biophysics

KW - polymers

KW - stoichiometry

KW - Computing and Processing

KW - Engineering Profession

KW - General Topics for Engineers

KW - Geoscience

KW - Nuclear Engineering

U2 - 10.1109/Transducers.2013.6626776

DO - 10.1109/Transducers.2013.6626776

M3 - Article in proceedings

SN - 9781467359832

BT - Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems

PB - IEEE

T2 - 17th International Conference on Solid-State Sensors, Actuators and Microsystems

Y2 - 16 June 2013 through 20 June 2013

ER -

Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers

Abstract

Conference

Keywords

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