Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers

Farizah Saharil, Annika Ahlford, Malte Kuhnemund, Maciej Skolimowski, Alvaro Conde, Martin Dufva, Mats Nilsson, Monica Brivio, Wouter van der Wijngaart, Tommy Haraldsson

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.
    Original languageEnglish
    Title of host publicationProceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems
    PublisherIEEE
    Publication date2013
    ISBN (Print)9781467359832
    DOIs
    Publication statusPublished - 2013
    Event17th International Conference on Solid-State Sensors, Actuators and Microsystems - Centro de Convenciones Internacional de Barcelona (CCIB) , Barcelona, Spain
    Duration: 16 Jun 201320 Jun 2013
    Conference number: 17
    http://transducers-eurosensors2013.org/

    Conference

    Conference17th International Conference on Solid-State Sensors, Actuators and Microsystems
    Number17
    LocationCentro de Convenciones Internacional de Barcelona (CCIB)
    CountrySpain
    CityBarcelona
    Period16/06/201320/06/2013
    Internet address

    Keywords

    • DNA
    • lab-on-a-chip
    • microfluidics
    • molecular biophysics
    • polymers
    • stoichiometry
    • Computing and Processing
    • Engineering Profession
    • General Topics for Engineers
    • Geoscience
    • Nuclear Engineering

    Cite this

    Saharil, F., Ahlford, A., Kuhnemund, M., Skolimowski, M., Conde, A., Dufva, M., Nilsson, M., Brivio, M., van der Wijngaart, W., & Haraldsson, T. (2013). Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. In Proceedings of the The 17th International Conference on Solid-State Sensors, Actuators and Microsystems IEEE. https://doi.org/10.1109/Transducers.2013.6626776