LC–MS/MS with atmospheric pressure chemical ionisation to study the effect of UV treatment on the formation of vitamin D3 and sterols in plants

Rie Bak Jäpelt, Daniele Silvestro, Jørn Smedsgaard, Poul Erik Jensen, Jette Jakobsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Some plant species are known to cause calcium intoxification in grazing animals. This has been attributed to the presence of vitamin D3-like activity. However, research into the presence of vitamin D3 in plants has been limited. One reason for this may be limitations in the analytical methods available for unambiguous detection and quantification of vitamin D3. This paper presents a new method for determining vitamin D3 and its sterol precursors. The method is based on saponification and extraction followed by solid phase clean-up of the compounds from plant leaves and detection by APCI-MS. Recoveries ranged from 101% to 114% and precision from 3% to 12%. Detection limits were 2–8ng/g fresh weight for the substances tested. In a pilot study we found that Solanum glaucohyllum Desf. and Solanum lycopersicum L. produced vitamin D3 after UV-treatment. The preliminary results presented suggest that vitamin D3 formation in plants is dependent on light exposure.
Original languageEnglish
JournalFood Chemistry
Volume129
Issue number1
Pages (from-to)217-225
ISSN0308-8146
DOIs
Publication statusPublished - 2011

Keywords

  • UV treatment
  • Vitamin D
  • APCI–LC–MS/MS
  • Sterols
  • Plants

Fingerprint

Dive into the research topics of 'LC–MS/MS with atmospheric pressure chemical ionisation to study the effect of UV treatment on the formation of vitamin D3 and sterols in plants'. Together they form a unique fingerprint.

Cite this