LC-MS/MS method for the determination of organophosphorus pesticides and their metabolites in salmon and zebrafish fed with plant-based feed ingredients

Borja Garlito, María Ibáñez, Tania Portolés, Roque Serrano, Heidi Amlund, Anne-Katrine Lundebye, Monica Sanden, Marc H G Berntssen, Félix Hernández*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The composition of Atlantic salmon feed has changed considerably over the last two decades from being marine-based (fishmeal and fish oil) to mainly containing plant ingredients. Consequently, concern related to traditional persistent contaminants typically associated with fish-based feed has been replaced by other potential contaminants not previously associated with salmon farming. This is the case for many pesticides, which are used worldwide to increase food production, and may be present in plant ingredients. Earlier studies have identified two organophosphorus pesticides, chlorpyrifos-methyl and pirimiphos-methyl, in plant ingredients used for aquafeed production. In the present study, we developed a reliable and sensitive analytical method, based on liquid chromatography coupled to tandem mass spectrometry, for the determination of these pesticides and their main metabolites in warm water (zebrafish) and cold water (Atlantic salmon) species, where possible differences in metabolites could be expected. The method was tested in whole zebrafish and in different salmon tissues, such as muscle, bile, kidney, fat, and liver. The final objective of this work was to assess kinetics of chlorpyrifos-methyl and pirimiphos-methyl and their main metabolites in fish tissue, in order to fill the knowledge gaps on these metabolites in fish tissues when fed over prolonged time.
Original languageEnglish
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number27
Pages (from-to)7281–7291
ISSN1618-2642
DOIs
Publication statusPublished - 2019

Keywords

  • Chlorpyrifos-methyl
  • Pirimiphos-methyl
  • Metabolites
  • Atlantic salmon
  • Zebrafish
  • LC-MS/MS

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