Large-Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems

Dávid Selmeczi; Thomas Steen Hansen; Özcan Met; Inge Marie Svane; Niels Bent Larsen

doi:10.1007/978-1-4939-3625-0_10

Large-Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems

Dávid Selmeczi
, Thomas Steen Hansen
, Özcan Met
, Inge Marie Svane
, Niels Bent Larsen

Copenhagen University Hospital Herlev and Gentofte

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Electroporation is well established for transient mRNA transfection of many mammalian cells, including immune cells such as dendritic cells used in cancer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synchronized electrical pulsing sequence can yield dendritic cell transfection rates of >75 % with survival rates of >90 %. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process.

Original language	English
Title of host publication	Synthetic mRNA : Production, Introduction Into Cells, and Physiological Consequences
Number of pages	11
Volume	1428
Publisher	Springer New York
Publication date	2016
Pages	151-61
Chapter	10
ISBN (Print)	978-1-4939-3623-6
ISBN (Electronic)	978-1-4939-3625-0
DOIs	https://doi.org/10.1007/978-1-4939-3625-0_10
Publication status	Published - 2016

Series	Methods in Molecular Biology
ISSN	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Dendritic cells
Electroporation
Laminar flow
Microfluidics
Transfection
mRNA

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10.1007/978-1-4939-3625-0_10

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abstract = "Electroporation is well established for transient mRNA transfection of many mammalian cells, including immune cells such as dendritic cells used in cancer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synchronized electrical pulsing sequence can yield dendritic cell transfection rates of >75 \% with survival rates of >90 \%. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process. ",

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Large-Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems. / Selmeczi, Dávid; Hansen, Thomas Steen; Met, Özcan et al.
Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences. Vol. 1428 Springer New York, 2016. p. 151-61 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Large-Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems

Abstract

UN SDGs

Keywords

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