Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1

Nadja Schultz-Jensen, Berith Elkær Knudsen, Zuzana Frkova, Jens Aamand, Tina Johansen, Jette Thykær, Sebastian R. Sørensen

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Abstract

The Aminobacter sp. strain MSH1 has potential for pesticide bioremediation because it degrades the herbicide metabolite 2,6-dichlorobenzamide (BAM). Production of the BAM-degrading bacterium using aerobic bioreactor fermentation was investigated. A mineral salt medium limited for carbon and with an element composition similar to the strain was generated. The optimal pH and temperature for strain growth were determined using shaker flasks and verified in bioreactors. Glucose, fructose, and glycerol were suitable carbon sources for MSH1 (μ =0.1 h−1); slower growth was observed on succinate and acetic acid (μ =0.01 h−1). Standard conditions for growth of theMSH1 strain were defined at pH 7 and 25 °C, with glucose as the carbon source. In bioreactors (1 and 5 L), the specific growth rate of MSH1 increased from μ =0.1 h−1 on traditional mineral salt medium to μ =0.18 h−1 on the optimized mineral salt medium. The biomass yield under standard conditions was 0.47 g dry weight biomass/g glucose consumed.An investigation of the catabolic capacity of MSH1 cells harvested in exponential and stationary growth phases showed a degradation activity per cell of about 3×10−9 μg BAM h−1. Thus, fast, efficient, large-scale production of herbicide-degrading Aminobacter was possible, bringing the use of this bacterium in bioaugmentation field remediation closer to reality.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume98
Issue number5
Pages (from-to)2335-2344
ISSN0175-7598
DOIs
Publication statusPublished - 2014

Cite this

Schultz-Jensen, N., Knudsen, B. E., Frkova, Z., Aamand, J., Johansen, T., Thykær, J., & Sørensen, S. R. (2014). Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1. Applied Microbiology and Biotechnology, 98(5), 2335-2344. https://doi.org/10.1007/s00253-013-5202-5
Schultz-Jensen, Nadja ; Knudsen, Berith Elkær ; Frkova, Zuzana ; Aamand, Jens ; Johansen, Tina ; Thykær, Jette ; Sørensen, Sebastian R. / Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1. In: Applied Microbiology and Biotechnology. 2014 ; Vol. 98, No. 5. pp. 2335-2344.
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abstract = "The Aminobacter sp. strain MSH1 has potential for pesticide bioremediation because it degrades the herbicide metabolite 2,6-dichlorobenzamide (BAM). Production of the BAM-degrading bacterium using aerobic bioreactor fermentation was investigated. A mineral salt medium limited for carbon and with an element composition similar to the strain was generated. The optimal pH and temperature for strain growth were determined using shaker flasks and verified in bioreactors. Glucose, fructose, and glycerol were suitable carbon sources for MSH1 (μ =0.1 h−1); slower growth was observed on succinate and acetic acid (μ =0.01 h−1). Standard conditions for growth of theMSH1 strain were defined at pH 7 and 25 °C, with glucose as the carbon source. In bioreactors (1 and 5 L), the specific growth rate of MSH1 increased from μ =0.1 h−1 on traditional mineral salt medium to μ =0.18 h−1 on the optimized mineral salt medium. The biomass yield under standard conditions was 0.47 g dry weight biomass/g glucose consumed.An investigation of the catabolic capacity of MSH1 cells harvested in exponential and stationary growth phases showed a degradation activity per cell of about 3×10−9 μg BAM h−1. Thus, fast, efficient, large-scale production of herbicide-degrading Aminobacter was possible, bringing the use of this bacterium in bioaugmentation field remediation closer to reality.",
author = "Nadja Schultz-Jensen and Knudsen, {Berith Elk{\ae}r} and Zuzana Frkova and Jens Aamand and Tina Johansen and Jette Thyk{\ae}r and S{\o}rensen, {Sebastian R.}",
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Schultz-Jensen, N, Knudsen, BE, Frkova, Z, Aamand, J, Johansen, T, Thykær, J & Sørensen, SR 2014, 'Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1', Applied Microbiology and Biotechnology, vol. 98, no. 5, pp. 2335-2344. https://doi.org/10.1007/s00253-013-5202-5

Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1. / Schultz-Jensen, Nadja; Knudsen, Berith Elkær; Frkova, Zuzana; Aamand, Jens; Johansen, Tina; Thykær, Jette; Sørensen, Sebastian R.

In: Applied Microbiology and Biotechnology, Vol. 98, No. 5, 2014, p. 2335-2344.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1

AU - Schultz-Jensen, Nadja

AU - Knudsen, Berith Elkær

AU - Frkova, Zuzana

AU - Aamand, Jens

AU - Johansen, Tina

AU - Thykær, Jette

AU - Sørensen, Sebastian R.

PY - 2014

Y1 - 2014

N2 - The Aminobacter sp. strain MSH1 has potential for pesticide bioremediation because it degrades the herbicide metabolite 2,6-dichlorobenzamide (BAM). Production of the BAM-degrading bacterium using aerobic bioreactor fermentation was investigated. A mineral salt medium limited for carbon and with an element composition similar to the strain was generated. The optimal pH and temperature for strain growth were determined using shaker flasks and verified in bioreactors. Glucose, fructose, and glycerol were suitable carbon sources for MSH1 (μ =0.1 h−1); slower growth was observed on succinate and acetic acid (μ =0.01 h−1). Standard conditions for growth of theMSH1 strain were defined at pH 7 and 25 °C, with glucose as the carbon source. In bioreactors (1 and 5 L), the specific growth rate of MSH1 increased from μ =0.1 h−1 on traditional mineral salt medium to μ =0.18 h−1 on the optimized mineral salt medium. The biomass yield under standard conditions was 0.47 g dry weight biomass/g glucose consumed.An investigation of the catabolic capacity of MSH1 cells harvested in exponential and stationary growth phases showed a degradation activity per cell of about 3×10−9 μg BAM h−1. Thus, fast, efficient, large-scale production of herbicide-degrading Aminobacter was possible, bringing the use of this bacterium in bioaugmentation field remediation closer to reality.

AB - The Aminobacter sp. strain MSH1 has potential for pesticide bioremediation because it degrades the herbicide metabolite 2,6-dichlorobenzamide (BAM). Production of the BAM-degrading bacterium using aerobic bioreactor fermentation was investigated. A mineral salt medium limited for carbon and with an element composition similar to the strain was generated. The optimal pH and temperature for strain growth were determined using shaker flasks and verified in bioreactors. Glucose, fructose, and glycerol were suitable carbon sources for MSH1 (μ =0.1 h−1); slower growth was observed on succinate and acetic acid (μ =0.01 h−1). Standard conditions for growth of theMSH1 strain were defined at pH 7 and 25 °C, with glucose as the carbon source. In bioreactors (1 and 5 L), the specific growth rate of MSH1 increased from μ =0.1 h−1 on traditional mineral salt medium to μ =0.18 h−1 on the optimized mineral salt medium. The biomass yield under standard conditions was 0.47 g dry weight biomass/g glucose consumed.An investigation of the catabolic capacity of MSH1 cells harvested in exponential and stationary growth phases showed a degradation activity per cell of about 3×10−9 μg BAM h−1. Thus, fast, efficient, large-scale production of herbicide-degrading Aminobacter was possible, bringing the use of this bacterium in bioaugmentation field remediation closer to reality.

U2 - 10.1007/s00253-013-5202-5

DO - 10.1007/s00253-013-5202-5

M3 - Journal article

VL - 98

SP - 2335

EP - 2344

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 5

ER -