As part of a collaboration with Danish Dairy Research Foundation we are interested in the DNA replication of Lactococcus lactis. For that we implemented flowcytometric analysis for these studies. The L. lactis does not respond to inhibition by rifampicin by finishing ongoing replication forks. We therefore turned to slow growing cultures in order to obtain information about the DNA replication in the cell cycle. From these studies we have obtained evidence that suggest that slow growing L. lactis are born with two chromosomes in contrast to other studied bacteria, which are born with one chromosome. This unexpected result has been confirmed by radioactive labelling of slow growing cultures of Lactococcus lactis, which also showed the presence of two chromosomes. We therefore conclude that Lactococcus lactis is the first diploid bacterium found.
|Publication status||Published - 2005|
|Event||Danish Food Science 2005 : From molecule to man - DTU|
Duration: 1 Jan 2005 → …
|Conference||Danish Food Science 2005 : From molecule to man|
|Period||01/01/2005 → …|