Lack of oestrogenic effects of food preservatives (parabens) in uterotrophic assays

A. Hossaini, Jens-Jørgen Larsen, John Christian Larsen

Research output: Contribution to journalJournal articleResearchpeer-review


The oestrogenic activity of the parabens, methyl-, ethyl- and propyl p-hydroxybenzoate, widely used as antimicrobials in food, and butyl p-hydroxybenzoate, which is used in cosmetic products, and their shared main metabolite p-hydroxybenzoic acid was investigated in a mouse uterotrophic assay, Immature B6D2F1 mice were treated with oral or subcutaneous doses of the test compounds for three consecutive days, p-Hydroxgybenzoic acid and butyl p-hydroxybenzoate were also tested by the subcutaneous route in a rat uterotrophic assay. A significant increase in the uterus weight at day 4 was considered an oestrogenic effect. In the mouse assay, none of the compounds tested produced any oestrogenic response at dose levels up to 100 mg/kg body weight per day, for ethyl p-hydroxybenzoate even at a dose level of 1000 mg/kg body weight per day. In immature Wistar rats, subcutaneous administration of butyl p-hydroxybenzoate produced a weak oestrogenic response at 600 mg/kg body weight per day.
Original languageEnglish
JournalFood and Chemical Toxicology
Issue number4
Pages (from-to)319-323
Publication statusPublished - 2000


  • mouse
  • parabens
  • oestrogen
  • rat
  • uterotrophic assay


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