Label-free protein detection using a microfluidic Coulter-counter device

Romén Rodriguez-Trujíllo, Mohammad Akram Ajine, A. Orzan, Mikkel Dysseholm Mar, F. Larsen, Casper Hyttel Clausen, Winnie Edith Svendsen

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    A new method for measuring specific protein concentrations in solutions has been developed. The technique makes use of the Coulter effect for detecting and sizing of micro-scaled objects suspended in a buffer fluid. The method is completely label-free as it is only based on the electrical readout when a suspension of microscopic beads flows over a set of electrodes in a microfluidic device. Since no electrode functionalization is needed the same device can be used in a number of different assays. Using goat-anti-rat IgG functionalized polystyrene beads we have shown proof of principle detecting rat IgG in solution. When the analyte (rat IgG) is present oligomers of beads are formed. The electrical readout of the oligomers is different compared to a zero control sample with no rat IgG. Detection of the protein has been performed in a concentration as small as 14 ng/mL. The dynamic range of the system has been demonstrated to be relatively large, ranging from 1 g/mL to 14 ng/mL. The microfluidic system is made from polymer and glass and very little volume of sample (<10 L) is needed for analysis.
    Original languageEnglish
    JournalSensors and Actuators B: Chemical
    Volume190
    Pages (from-to)922-927
    ISSN0925-4005
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Label-free detection
    • Protein
    • Coulter-counter
    • Microfluidics
    • Impedance
    • Bead-based assay

    Cite this

    Rodriguez-Trujíllo, Romén ; Ajine, Mohammad Akram ; Orzan, A. ; Mar, Mikkel Dysseholm ; Larsen, F. ; Clausen, Casper Hyttel ; Svendsen, Winnie Edith. / Label-free protein detection using a microfluidic Coulter-counter device. In: Sensors and Actuators B: Chemical. 2014 ; Vol. 190. pp. 922-927.
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    abstract = "A new method for measuring specific protein concentrations in solutions has been developed. The technique makes use of the Coulter effect for detecting and sizing of micro-scaled objects suspended in a buffer fluid. The method is completely label-free as it is only based on the electrical readout when a suspension of microscopic beads flows over a set of electrodes in a microfluidic device. Since no electrode functionalization is needed the same device can be used in a number of different assays. Using goat-anti-rat IgG functionalized polystyrene beads we have shown proof of principle detecting rat IgG in solution. When the analyte (rat IgG) is present oligomers of beads are formed. The electrical readout of the oligomers is different compared to a zero control sample with no rat IgG. Detection of the protein has been performed in a concentration as small as 14 ng/mL. The dynamic range of the system has been demonstrated to be relatively large, ranging from 1 g/mL to 14 ng/mL. The microfluidic system is made from polymer and glass and very little volume of sample (<10 L) is needed for analysis.",
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    Label-free protein detection using a microfluidic Coulter-counter device. / Rodriguez-Trujíllo, Romén; Ajine, Mohammad Akram; Orzan, A.; Mar, Mikkel Dysseholm; Larsen, F.; Clausen, Casper Hyttel; Svendsen, Winnie Edith.

    In: Sensors and Actuators B: Chemical, Vol. 190, 2014, p. 922-927.

    Research output: Contribution to journalJournal articleResearchpeer-review

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    AU - Rodriguez-Trujíllo, Romén

    AU - Ajine, Mohammad Akram

    AU - Orzan, A.

    AU - Mar, Mikkel Dysseholm

    AU - Larsen, F.

    AU - Clausen, Casper Hyttel

    AU - Svendsen, Winnie Edith

    PY - 2014

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